V3306

antibody from NSJ Bioreagents

Targeting: GJB1 CMTX, CMTX1, CX32

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V3306

Provider

NSJ Bioreagents

Product name

Connexin 32 Antibody / GJB1

Antibody type

Monoclonal

Description

Purified

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

M12.13

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)

Concentration

0.2 mg/ml

Storage

Store the Connexin 32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

References

Submitted references

Retinoids regulate the formation and degradation of gap junctions in androgen-responsive human prostate cancer cells.

Kelsey L, Katoch P, Johnson KE, Batra SK, Mehta PP
PloS one 2012;7(4):e32846

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E-cadherin differentially regulates the assembly of Connexin43 and Connexin32 into gap junctions in human squamous carcinoma cells.

Chakraborty S, Mitra S, Falk MM, Caplan SH, Wheelock MJ, Johnson KR, Mehta PP
The Journal of biological chemistry 2010 Apr 2;285(14):10761-76

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Regulation of ubiquitin-proteasome system mediated degradation by cytosolic stress.

Kelly SM, Vanslyke JK, Musil LS
Molecular biology of the cell 2007 Nov;18(11):4279-91

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2-aminoethoxydiphenyl borate directly inhibits channels composed of connexin26 and/or connexin32.

Tao L, Harris AL
Molecular pharmacology 2007 Feb;71(2):570-9

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Isoelectric points and post-translational modifications of connexin26 and connexin32.

Locke D, Koreen IV, Harris AL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 Jun;20(8):1221-3

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Heteromeric, but not homomeric, connexin channels are selectively permeable to inositol phosphates.

Ayad WA, Locke D, Koreen IV, Harris AL
The Journal of biological chemistry 2006 Jun 16;281(24):16727-39

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The effects of a dominant connexin32 mutant in myelinating Schwann cells.

Jeng LJ, Balice-Gordon RJ, Messing A, Fischbeck KH, Scherer SS
Molecular and cellular neurosciences 2006 Jul;32(3):283-98

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Androgen-regulated formation and degradation of gap junctions in androgen-responsive human prostate cancer cells.

Mitra S, Annamalai L, Chakraborty S, Johnson K, Song XH, Batra SK, Mehta PP
Molecular biology of the cell 2006 Dec;17(12):5400-16

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Lipid rafts prepared by different methods contain different connexin channels, but gap junctions are not lipid rafts.

Locke D, Liu J, Harris AL
Biochemistry 2005 Oct 4;44(39):13027-42

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Subcellular localization of disease-associated prion protein in the human brain.

Kovács GG, Preusser M, Strohschneider M, Budka H
The American journal of pathology 2005 Jan;166(1):287-94

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Biochemical requirements for inhibition of Connexin26-containing channels by natural and synthetic taurine analogs.

Tao L, Harris AL
The Journal of biological chemistry 2004 Sep 10;279(37):38544-54

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Analysis of protein expression in brain tissue by ELISA.

Bennett SA, Roberts DC
Methods in molecular medicine 2003;79:283-95

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Impaired trafficking of connexins in androgen-independent human prostate cancer cell lines and its mitigation by alpha-catenin.

Govindarajan R, Zhao S, Song XH, Guo RJ, Wheelock M, Johnson KR, Mehta PP
The Journal of biological chemistry 2002 Dec 20;277(51):50087-97

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Phenotypes of X-linked Charcot-Marie-Tooth disease and altered trafficking of mutant connexin 32 (GJB1).

Matsuyama W, Nakagawa M, Moritoyo T, Takashima H, Umehara F, Hirata K, Suehara M, Osame M
Journal of human genetics 2001;46(6):307-13

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Interruption of hepatic gap junctional communication in the rat during inflammation induced by bacterial lipopolysaccharide.

De Maio A, Gingalewski C, Theodorakis NG, Clemens MG
Shock (Augusta, Ga.) 2000 Jul;14(1):53-9

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Long-term changes in connexin32 gap junction protein and mRNA expression following cocaine self-administration in rats.

Bennett SA, Arnold JM, Chen J, Stenger J, Paul DL, Roberts DC
The European journal of neuroscience 1999 Sep;11(9):3329-38

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Direct high affinity modulation of connexin channel activity by cyclic nucleotides.

Bevans CG, Harris AL
The Journal of biological chemistry 1999 Feb 5;274(6):3720-5

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Connexin32-null mice develop demyelinating peripheral neuropathy.

Scherer SS, Xu YT, Nelles E, Fischbeck K, Willecke K, Bone LJ
Glia 1998 Sep;24(1):8-20

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Connexin32 mutations associated with X-linked Charcot-Marie-Tooth disease show two distinct behaviors: loss of function and altered gating properties.

Ressot C, Gomès D, Dautigny A, Pham-Dinh D, Bruzzone R
The Journal of neuroscience : the official journal of the Society for Neuroscience 1998 Jun 1;18(11):4063-75

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Isoform composition of connexin channels determines selectivity among second messengers and uncharged molecules.

Bevans CG, Kordel M, Rhee SK, Harris AL
The Journal of biological chemistry 1998 Jan 30;273(5):2808-16

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Proliferation-associated differences in the spatial and temporal expression of gap junction genes in rat liver.

Neveu MJ, Hully JR, Babcock KL, Vaughan J, Hertzberg EL, Nicholson BJ, Paul DL, Pitot HC
Hepatology (Baltimore, Md.) 1995 Jul;22(1):202-12

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Connexin32 is a myelin-related protein in the PNS and CNS.

Scherer SS, Deschênes SM, Xu YT, Grinspan JB, Fischbeck KH, Paul DL
The Journal of neuroscience : the official journal of the Society for Neuroscience 1995 Dec;15(12):8281-94

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE Analysis of Purified, BSA-Free Connexin 32 Antibody (clone M12.13). Confirmation of Integrity and Purity of the Antibody.