Antibody data
- Antibody Data
- Antigen structure
- References [22]
- Comments [0]
- Validations
- Other assay [1]
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- Product number
- V3306 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Connexin 32 Antibody / GJB1
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- M12.13
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml
- Storage
- Store the Connexin 32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Retinoids regulate the formation and degradation of gap junctions in androgen-responsive human prostate cancer cells.
E-cadherin differentially regulates the assembly of Connexin43 and Connexin32 into gap junctions in human squamous carcinoma cells.
Regulation of ubiquitin-proteasome system mediated degradation by cytosolic stress.
2-aminoethoxydiphenyl borate directly inhibits channels composed of connexin26 and/or connexin32.
Isoelectric points and post-translational modifications of connexin26 and connexin32.
Heteromeric, but not homomeric, connexin channels are selectively permeable to inositol phosphates.
The effects of a dominant connexin32 mutant in myelinating Schwann cells.
Androgen-regulated formation and degradation of gap junctions in androgen-responsive human prostate cancer cells.
Lipid rafts prepared by different methods contain different connexin channels, but gap junctions are not lipid rafts.
Subcellular localization of disease-associated prion protein in the human brain.
Biochemical requirements for inhibition of Connexin26-containing channels by natural and synthetic taurine analogs.
Analysis of protein expression in brain tissue by ELISA.
Impaired trafficking of connexins in androgen-independent human prostate cancer cell lines and its mitigation by alpha-catenin.
Phenotypes of X-linked Charcot-Marie-Tooth disease and altered trafficking of mutant connexin 32 (GJB1).
Interruption of hepatic gap junctional communication in the rat during inflammation induced by bacterial lipopolysaccharide.
Long-term changes in connexin32 gap junction protein and mRNA expression following cocaine self-administration in rats.
Direct high affinity modulation of connexin channel activity by cyclic nucleotides.
Connexin32-null mice develop demyelinating peripheral neuropathy.
Connexin32 mutations associated with X-linked Charcot-Marie-Tooth disease show two distinct behaviors: loss of function and altered gating properties.
Isoform composition of connexin channels determines selectivity among second messengers and uncharged molecules.
Proliferation-associated differences in the spatial and temporal expression of gap junction genes in rat liver.
Connexin32 is a myelin-related protein in the PNS and CNS.
Kelsey L, Katoch P, Johnson KE, Batra SK, Mehta PP
PloS one 2012;7(4):e32846
PloS one 2012;7(4):e32846
E-cadherin differentially regulates the assembly of Connexin43 and Connexin32 into gap junctions in human squamous carcinoma cells.
Chakraborty S, Mitra S, Falk MM, Caplan SH, Wheelock MJ, Johnson KR, Mehta PP
The Journal of biological chemistry 2010 Apr 2;285(14):10761-76
The Journal of biological chemistry 2010 Apr 2;285(14):10761-76
Regulation of ubiquitin-proteasome system mediated degradation by cytosolic stress.
Kelly SM, Vanslyke JK, Musil LS
Molecular biology of the cell 2007 Nov;18(11):4279-91
Molecular biology of the cell 2007 Nov;18(11):4279-91
2-aminoethoxydiphenyl borate directly inhibits channels composed of connexin26 and/or connexin32.
Tao L, Harris AL
Molecular pharmacology 2007 Feb;71(2):570-9
Molecular pharmacology 2007 Feb;71(2):570-9
Isoelectric points and post-translational modifications of connexin26 and connexin32.
Locke D, Koreen IV, Harris AL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 Jun;20(8):1221-3
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 Jun;20(8):1221-3
Heteromeric, but not homomeric, connexin channels are selectively permeable to inositol phosphates.
Ayad WA, Locke D, Koreen IV, Harris AL
The Journal of biological chemistry 2006 Jun 16;281(24):16727-39
The Journal of biological chemistry 2006 Jun 16;281(24):16727-39
The effects of a dominant connexin32 mutant in myelinating Schwann cells.
Jeng LJ, Balice-Gordon RJ, Messing A, Fischbeck KH, Scherer SS
Molecular and cellular neurosciences 2006 Jul;32(3):283-98
Molecular and cellular neurosciences 2006 Jul;32(3):283-98
Androgen-regulated formation and degradation of gap junctions in androgen-responsive human prostate cancer cells.
Mitra S, Annamalai L, Chakraborty S, Johnson K, Song XH, Batra SK, Mehta PP
Molecular biology of the cell 2006 Dec;17(12):5400-16
Molecular biology of the cell 2006 Dec;17(12):5400-16
Lipid rafts prepared by different methods contain different connexin channels, but gap junctions are not lipid rafts.
Locke D, Liu J, Harris AL
Biochemistry 2005 Oct 4;44(39):13027-42
Biochemistry 2005 Oct 4;44(39):13027-42
Subcellular localization of disease-associated prion protein in the human brain.
Kovács GG, Preusser M, Strohschneider M, Budka H
The American journal of pathology 2005 Jan;166(1):287-94
The American journal of pathology 2005 Jan;166(1):287-94
Biochemical requirements for inhibition of Connexin26-containing channels by natural and synthetic taurine analogs.
Tao L, Harris AL
The Journal of biological chemistry 2004 Sep 10;279(37):38544-54
The Journal of biological chemistry 2004 Sep 10;279(37):38544-54
Analysis of protein expression in brain tissue by ELISA.
Bennett SA, Roberts DC
Methods in molecular medicine 2003;79:283-95
Methods in molecular medicine 2003;79:283-95
Impaired trafficking of connexins in androgen-independent human prostate cancer cell lines and its mitigation by alpha-catenin.
Govindarajan R, Zhao S, Song XH, Guo RJ, Wheelock M, Johnson KR, Mehta PP
The Journal of biological chemistry 2002 Dec 20;277(51):50087-97
The Journal of biological chemistry 2002 Dec 20;277(51):50087-97
Phenotypes of X-linked Charcot-Marie-Tooth disease and altered trafficking of mutant connexin 32 (GJB1).
Matsuyama W, Nakagawa M, Moritoyo T, Takashima H, Umehara F, Hirata K, Suehara M, Osame M
Journal of human genetics 2001;46(6):307-13
Journal of human genetics 2001;46(6):307-13
Interruption of hepatic gap junctional communication in the rat during inflammation induced by bacterial lipopolysaccharide.
De Maio A, Gingalewski C, Theodorakis NG, Clemens MG
Shock (Augusta, Ga.) 2000 Jul;14(1):53-9
Shock (Augusta, Ga.) 2000 Jul;14(1):53-9
Long-term changes in connexin32 gap junction protein and mRNA expression following cocaine self-administration in rats.
Bennett SA, Arnold JM, Chen J, Stenger J, Paul DL, Roberts DC
The European journal of neuroscience 1999 Sep;11(9):3329-38
The European journal of neuroscience 1999 Sep;11(9):3329-38
Direct high affinity modulation of connexin channel activity by cyclic nucleotides.
Bevans CG, Harris AL
The Journal of biological chemistry 1999 Feb 5;274(6):3720-5
The Journal of biological chemistry 1999 Feb 5;274(6):3720-5
Connexin32-null mice develop demyelinating peripheral neuropathy.
Scherer SS, Xu YT, Nelles E, Fischbeck K, Willecke K, Bone LJ
Glia 1998 Sep;24(1):8-20
Glia 1998 Sep;24(1):8-20
Connexin32 mutations associated with X-linked Charcot-Marie-Tooth disease show two distinct behaviors: loss of function and altered gating properties.
Ressot C, Gomès D, Dautigny A, Pham-Dinh D, Bruzzone R
The Journal of neuroscience : the official journal of the Society for Neuroscience 1998 Jun 1;18(11):4063-75
The Journal of neuroscience : the official journal of the Society for Neuroscience 1998 Jun 1;18(11):4063-75
Isoform composition of connexin channels determines selectivity among second messengers and uncharged molecules.
Bevans CG, Kordel M, Rhee SK, Harris AL
The Journal of biological chemistry 1998 Jan 30;273(5):2808-16
The Journal of biological chemistry 1998 Jan 30;273(5):2808-16
Proliferation-associated differences in the spatial and temporal expression of gap junction genes in rat liver.
Neveu MJ, Hully JR, Babcock KL, Vaughan J, Hertzberg EL, Nicholson BJ, Paul DL, Pitot HC
Hepatology (Baltimore, Md.) 1995 Jul;22(1):202-12
Hepatology (Baltimore, Md.) 1995 Jul;22(1):202-12
Connexin32 is a myelin-related protein in the PNS and CNS.
Scherer SS, Deschênes SM, Xu YT, Grinspan JB, Fischbeck KH, Paul DL
The Journal of neuroscience : the official journal of the Society for Neuroscience 1995 Dec;15(12):8281-94
The Journal of neuroscience : the official journal of the Society for Neuroscience 1995 Dec;15(12):8281-94
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Supportive validation
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- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Connexin 32 Antibody (clone M12.13). Confirmation of Integrity and Purity of the Antibody.