14-9759-80

antibody from Invitrogen Antibodies

Targeting: GJB1 CMTX, CMTX1, CX32

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

14-9759-80

Provider

Invitrogen Antibodies

Product name

Anti-Connexin 32 Monoclonal Antibody (Connexin32 (2A)), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The Connexin32 monoclonal antibody recognizes human, mouse, rat, and chicken Connexin 32. Connexins are a family of integral membrane proteins that form gap junction channels, which function to facilitate direct cell-cell communication. Gap junctions are comprised of two hemichannels, or connexons, which are made up of six connexin proteins. Over 20 connexin proteins have been identified and each combine to form either homomeric or heteromeric channels with different functional properties. Connexin 32 is expressed by myelin, neurons, oligodendrocytes, and hepatocytes, and plays a role in the development of ovarian follicles. Connexin 32 expression also plays a role in disease progression in chronic multiple sclerosis and experimental autoimmune encephalomyelitis. Mutations in the Connexin 32 gene, GJB1, result in a form of Charcot-Marie-Tooth disease which is characterized by peripheral nerve dysfunction. Applications Reported: This Connexin32 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. Applications Tested: This Connexin32 antibody has been tested by immunohistochemistry of frozen mouse tissue and immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and the antibody can be used at less than or equal to 5 µg/mL. This Connexin32 antibody has been tested by immunocytochemistry of methanol-fixed and permeabilized human cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human, Mouse, Rat, Chicken/Avian

Host

Mouse

Isotype

IgG

Antibody clone number

Connexin32 (2A)

Vial size

25 µg

Concentration

0.5 mg/mL

Storage

4° C

References

Submitted references

The liver connexin32 interactome is a novel plasma membrane-mitochondrial signaling nexus.

Fowler SL, Akins M, Zhou H, Figeys D, Bennett SA
Journal of proteome research 2013 Jun 7;12(6):2597-610

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Functional requirement for a highly conserved charged residue at position 75 in the gap junction protein connexin 32.

Abrams CK, Islam M, Mahmoud R, Kwon T, Bargiello TA, Freidin MM
The Journal of biological chemistry 2013 Feb 1;288(5):3609-19

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Stage-specific and differential expression of gap junctions in the mouse ovary: connexin-specific roles in follicular regulation.

Wright CS, Becker DL, Lin JS, Warner AE, Hardy K
Reproduction (Cambridge, England) 2001 Jan;121(1):77-88

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of frozen mouse liver using 5 µg/mL of Anti-Connexin 32 Purified followed by 5 µg/mL of F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of formalin-fixed paraffin embedded human liver using 5 µg/mL of Anti-Connexin 32 Purified, followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.