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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunohistochemistry [2]
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- Product number
- 14-9759-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Connexin 32 Monoclonal Antibody (Connexin32 (2A)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The Connexin32 monoclonal antibody recognizes human, mouse, rat, and chicken Connexin 32. Connexins are a family of integral membrane proteins that form gap junction channels, which function to facilitate direct cell-cell communication. Gap junctions are comprised of two hemichannels, or connexons, which are made up of six connexin proteins. Over 20 connexin proteins have been identified and each combine to form either homomeric or heteromeric channels with different functional properties. Connexin 32 is expressed by myelin, neurons, oligodendrocytes, and hepatocytes, and plays a role in the development of ovarian follicles. Connexin 32 expression also plays a role in disease progression in chronic multiple sclerosis and experimental autoimmune encephalomyelitis. Mutations in the Connexin 32 gene, GJB1, result in a form of Charcot-Marie-Tooth disease which is characterized by peripheral nerve dysfunction.
- Antibody clone number
- Connexin32 (2A)
- Concentration
- 0.5 mg/mL
Submitted references The liver connexin32 interactome is a novel plasma membrane-mitochondrial signaling nexus.
Functional requirement for a highly conserved charged residue at position 75 in the gap junction protein connexin 32.
Stage-specific and differential expression of gap junctions in the mouse ovary: connexin-specific roles in follicular regulation.
Fowler SL, Akins M, Zhou H, Figeys D, Bennett SA
Journal of proteome research 2013 Jun 7;12(6):2597-610
Journal of proteome research 2013 Jun 7;12(6):2597-610
Functional requirement for a highly conserved charged residue at position 75 in the gap junction protein connexin 32.
Abrams CK, Islam M, Mahmoud R, Kwon T, Bargiello TA, Freidin MM
The Journal of biological chemistry 2013 Feb 1;288(5):3609-19
The Journal of biological chemistry 2013 Feb 1;288(5):3609-19
Stage-specific and differential expression of gap junctions in the mouse ovary: connexin-specific roles in follicular regulation.
Wright CS, Becker DL, Lin JS, Warner AE, Hardy K
Reproduction (Cambridge, England) 2001 Jan;121(1):77-88
Reproduction (Cambridge, England) 2001 Jan;121(1):77-88
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of frozen mouse liver using 5 µg/mL of Anti-Connexin 32 Purified followed by 5 µg/mL of F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human liver using 5 µg/mL of Anti-Connexin 32 Purified, followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
