PA5-107277

antibody from Invitrogen Antibodies

Targeting: SPRR1B GADD33, SPRR1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-107277

Provider

Invitrogen Antibodies

Product name

SPRR1B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Sequence homology: Cow: 85%; Horse: 100%; Human: 100%; Mouse: 85%; Pig: 92%; Rabbit: 92%; Rat: 85%

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SPRR1B in human Jurkat cells using a SPRR1B Polyclonal Antibody (Product # PA5-107277) diluted to 1 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of SPRR1B in mouse eye section using a SPRR1B Polyclonal Antibody (Product # PA5-107277) diluted to 1:50. Secondary antibody: Goat anti-rabbit-AlexaFluor 488, diluted 1:50.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Surface Plasmon Resonance of SPRR1B polyclonal antibody (Product # PA5-107277). Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip at concentrations of 5, and 50 µg/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333 nm peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.