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LearnRQ7746
antibody from NSJ Bioreagents
Targeting: SYNE3
C14orf139, C14orf49, FLJ25605, LINC00341, NCRNA00341, Nesp3, Nesprin-3, NET53
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- RQ7746 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Nesprin 3 Antibody / SYNE3
- Antibody type
- Polyclonal
- Description
- This highly specific Nesprin 3 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Direct ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Nesprin 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) 293T and 2) HeLa cell lysate with Nesprin 3 antibody. Predicted molecular weight ~112 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver cancer tissue with Nesprin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human mouse liver tissue with Nesprin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human spleen rupture tissue with Nesprin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human thyroid papillary carcinoma tissue with Nesprin 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-87 MG cells with Nesprin 3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nesprin 3 antibody.
