PA5-87873

antibody from Invitrogen Antibodies

Targeting: GNA14

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87873

Provider

Invitrogen Antibodies

Product name

GNA14 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LLGTGESGKS TFIKQMRIIH GSGYSDEDRK GFTKLVYQNI FTAMQAMIRA MDTLRIQYVC EQNKENAQII REVEVDKVSM LSREQVEAIK QLWQDPGIQE CYDRRREYQL SDSAKYYLTD IDRIATPSFV PTQQDVLRVR VPTTGIIEYP FDLENIIFRM VDVGGQRSER RKWIHCFESV TSIIFLVALS EYDQVLAECD NENRMEESKA LFKTIITYPW FLNSSVILFL NKKDLLEEKI MYSHLISYFP EYTGPKQDVR AARDFILKLY QDQNPDKEKV IYSHFTCATD TDNIRFVFAA VKDTILQLNL R; Positive Samples: BxPC-3, 293T, Mouse stomach, Mouse lung, Rat kidney

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.73 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GNA14 in extracts of various cell lines. Samples were incubated with GNA14 Polyclonal antibody (Product # PA5-87873) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GNA14 was performed in NIH/3T3 cells using GNA14 Polyclonal Antibody (Product # PA5-87873) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GNA14 was performed in NIH/3T3 cells using GNA14 Polyclonal Antibody (Product # PA5-87873) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GNA14 in NIH/3T3 cells. Samples were incubated with GNA14 Polyclonal antibody (Product # PA5-87873) using a dilution of 1:100. Blue: DAPI for nuclear staining.