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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- ANX-012-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCX2 (SLC8A2) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat skeletal muscle lysate (lanes 3 and 7) and human SH-SY5Y neuroblastoma cell lysate (lanes 4 and 8): - 1-4. Anti-NCX2 (SLC8A2) Antibody (#ANX-012), (1:200).5-8. Anti-NCX2 (SLC8A2) Antibody , preincubated with NCX2/SLC8A2 Blocking Peptide (#BLP-NX012).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of NCX2 in rat cerebellum - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NCX2 (SLC8A2) Antibody (#ANX-012), (1:100). A. NCX2 staining (red) is expressed in the molecular layer (M) and Purkinje cells (arrows). B. DAPI counterstain (blue) displays the layout of cerebellar layers. C. Merge of A and B demonstrates restriction of NCX2 expression to the molecular layer and Purkinje cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of NCX2 in rat cerebellum - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NCX2 (SLC8A2) Antibody (#ANX-012), (1:100). A. NCX2 staining (red) is expressed in the molecular layer (M) and Purkinje cells (arrows). B. DAPI counterstain (blue) displays the layout of cerebellar layers. C. Merge of A and B demonstrates restriction of NCX2 expression to the molecular layer and Purkinje cells.
