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Immunocytochemistry
Blocking/Neutralizing
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- 711912 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H1.5 Recombinant Superclonal™ Antibody (16HCLC)
- Antibody type
- Other
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with Monkey, Cat, Rat. Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 16HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis,Ntera-2 cells were fixed and permiabilized for detection of endogenous Histone H1.5 using Anti-Histone H1.5 Recombinant Rabbit Superclonal™ Antibody (Product # 711912, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Histone H1.5 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938, 1:50). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panel a, b and c clearly demonstrating nuclear localization of Histone H1.5 Panel e) represents control cells with no primary Antibody to assess background.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis,Ntera-2 cells were fixed and permiabilized for detection of endogenous Histone H1.5 using Anti-Histone H1.5 Recombinant Rabbit Superclonal™ Antibody (Product # 711912, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Histone H1.5 protein (green). Antibody specificity was demonstrated by competition with the H1.5 peptide, which results in loss of signal (Panel e). No competition was observed with the H1.2, H1.3 and H1.4 peptides (b,c,d). The images were captured at 60X magnification.
