PA5-80862

antibody from Invitrogen Antibodies

Targeting: NOXRED1 C14orf148, FLJ32809

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80862

Provider

Invitrogen Antibodies

Product name

NOXRED1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NOXRED1 in Lane A: HT1080 Whole Cell Lysate (30 µg). Samples were probed using a NOXRED1 Polyclonal Antibody (Product # PA5-80862) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:40 kDa. Observed band size:40 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using NOXRED1 Polyclonal Antibody (Product # PA5-80862) at 1:500 dilution. Lane A: HT1080 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa. (We are unsure of the identity of these extra bands).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NOXRED1 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 µL with NOXRED1 Polyclonal Antibody (Product # PA5-80862) and 15 µL of 50 % Protein G agarose. Primary antibody: NOXRED1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 40 kDa.