14797-1-AP

antibody from Invitrogen Antibodies

Targeting: HMBS PBGD, PORC, UPS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14797-1-AP

Provider

Invitrogen Antibodies

Product name

HMBS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSGNGNAAA TAEENSPKMR VIRVGTRKSQ LARIQTDSVV ATLKASYPGL QFEIIAMSTT GDKILDTALS KIGEKSLFTK ELEHALEKNE VDLVVHSLKD LPTVLPPGFT IGAICKRENP HDAVVFHPKF VGKTLETLPE KSVVGTSSLR RAAQLQRKFP HLEFRSIRGN LNTRLRKLDE QQEFSAIILA TAGLQRMGWH NRVGQILHPE ECMYAVGQGA LGVEVRAKDQ DILDLVGVLH DPETLLRCIA ERAFLRHLEG GCSVPVAVHT AMKDGQLYLT GGVWSLDGSD SIQETMQATI HVPAQHEDGP EDDPQLVGIT ARNIPRGPQL AAQNLGISLA NLLLSKGAKN ILDVARQLND AH (1-361 aa encoded by BC000520)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.2 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Raji cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 14797-1-AP (HMBS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using HMBS antibody 14797-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using HMBS antibody 14797-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using HMBS antibody 14797-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-HMBS (IP:14797-1-AP, 4ug; Detection:14797-1-AP 1:700) with HEK-293 cells lysate 2400 ug.