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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [11]
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- Product number
- UM800041 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HMBS Monoclonal Antibody (UMAB144), UltraMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- UMAB144
- Vial size
- 100 µL
- Concentration
- 0.5-1.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15 µg) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Dilution: 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KM12 cell lysate (35 µg) by using anti-HMBS monoclonal antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-HMBS mouse monoclonal antibody (UM800041, green, 1:50). Actin filaments were labeled with Alexa Fluor 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human breast tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human tonsil using anti-HMBS clone UMAB144 mouse monoclonal antibody at 1:400 with Polink2 Broad HRP DAB detection kit (UM800041); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Strong cytoplasmic and membraneous staining is seen on tonsil germinal and non-germinal centers. Some weak nuclear stain was also observed.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human prostate cancer using anti-HMBS clone UMAB144 mouse monoclonal antibody at 1:400 with Polink2 Broad HRP DAB detection kit (UM800041); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Weak nuclear staining is seen on tumor cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human thyroid tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human lymph node tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human muscle tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human embryonic brain cortex tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human embryonic cerebellum using anti-HMBSmouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human Kidney tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HMBS mouse monoclonal antibody. (UM800041; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)
