MA5-24155

antibody from Invitrogen Antibodies

Targeting: GPR34

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24155

Provider

Invitrogen Antibodies

Product name

GPR34 Monoclonal Antibody (419859)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody stains human GPR34 transfectants but not irrelevant transfectants.

Antibody clone number

419859

Concentration

0.5 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of GPR34 in immersion fixed A172 human glioblastoma cell line. Samples were incubated in GPR34 monoclonal antibody (Product # MA5-24155) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of A172 human glioblastoma cell line was stained with mouse Anti-human GPR34 Monoclonal Antibody (Product # MA5-24155) or isotype control antibodyopen histogram), followed by Phycoerythrin-conjugated Anti-mouse IgG F (ab')2 Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of GPR34 in A172 human glioblastoma cell line. Samples were incubated in GPR34 monoclonal antibody (Product # MA5-24155) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.