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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- GTX78182 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX78182, RRID:AB_625833
- Product name
- VR1/ TRPV1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Rat
- Host
- Rabbit
- Storage
- Upon Receipt - Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- (C) TRPV1 in HEK293 cells was not tyrosine phosphorylated. HEK293 cells expressing combinations of TRPV1, p75, and trkA, as indicated in the figure, were treated with either NGF or vehicle and then immunoprecipitated with anti-trkA (lanes 1¡V7) or anti-TRPV1 (lanes 8¡V13) antibodies. Lanes 1¡V7 were separated from lanes 8¡V13 and the membranes were probed with anti-trkA antibody and anti-TRPV1 antibody, respectively (top). Segments of the membrane were then reapposed for imaging. The membranes were then stripped and reprobed with anti-phosphotyrosine antibody (bottom)
