GTX80798

antibody from GeneTex

Targeting: TRPV1 VR1

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX80798

Provider

GeneTex

Proper citation

GeneTex Cat#GTX80798, RRID:AB_625832

Product name

VR1/ TRPV1 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Guinea Pig

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

TRPV1 staining of mouse inferior olive using Cy3-conjugated donkey anti-rabbit secondary antibodies (red color) and DAPI (blue) as a nuclear counterstain.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

VR1-C staining of rat dorsal horn (dilution 1:100).

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Evaluation of the expression patterns of TLR4 and CD14 in trigeminal sensory neurons. White arrows depict examples of neurons expressing both markers for each row of three images, and yellow arrows depict examples of neurons that express one but not both markers. Human trigeminal neurons were evaluated for co-localization of TLR4 (Panels A,D), CD14 (Panel B), with a marker for the capsaicinsensitive subclass of nociceptors (TRPV1, Panels B,C for TLR4 and Panels K,L for CD14), or a marker of myelinated sensory neurons (N52, Panels Figure 1. Evaluation of the expression patterns of TLR4 and CD14 in trigeminal sensory neurons. White arrows depict examples of neurons expressing both markers for each row of three images, and yellow arrows depict examples of neurons that express one but not both markers. Human trigeminal neurons were evaluated for co-localization of TLR4 (Panels A,D), CD14 (Panel J), with a marker for the capsaicinsensitive subclass of nociceptors (TRPV1, Panels B,C for TLR4 and Panels K,L for CD14), or a marker of myelinated sensory neurons (N52, Panels E,F). Rat trigeminal neurons were evaluated for co-localization of TLR4 with TRPV1 (Panels G-I) and CD14 with TRPV1 (Panels M-O). The addition of blocking peptide or the deletion of primary or secondary antisera produced a complete loss of signal in both the human and rat tissues. Scale bar is 100 um for Panels A-F and J-L, and 200 um for Panels G-I and M-O. Rat trigeminal neurons were evaluated for co-localization of TLR4 with TRPV1 (Panels G-I) and CD14 with TRPV1 (Panels M-O). The addition of blocking peptide or the deletion of primary or secondary antisera produced a complete loss of signal in both the human and rat tissues. Scale bar is 100 um for Panels A-F and J-L, and 200 um for Panels G-I and M-O