PA5-120401

antibody from Invitrogen Antibodies

Targeting: ELSPBP1 E12, EDDM12, HE12

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120401

Provider

Invitrogen Antibodies

Product name

ELSPBP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.86 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ELSPBP1 in various cell lines using a ELSPBP1 Polyclonal antibody (Product # PA5-120401) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 90s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ELSPBP1 in extracts of various cell lines. Samples were incubated with ELSPBP1 Polyclonal antibody (Product # PA5-120401) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of ELSPBP1 in mouse testis using a ELSPBP1 Polyclonal antibody (Product # PA5-120401). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Immunofluorescence) analysis of ELSPBP1 in mouse testis. Samples were incubated with ELSPBP1 Polyclonal antibody (Product # PA5-120401) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Immunofluorescence) analysis of ELSPBP1 in rat testis. Samples were incubated with ELSPBP1 Polyclonal antibody (Product # PA5-120401) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Immunofluorescence) analysis of ELSPBP1 in mouse testis. Samples were incubated with ELSPBP1 Polyclonal antibody (Product # PA5-120401) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Immunofluorescence) analysis of ELSPBP1 in rat testis. Samples were incubated with ELSPBP1 Polyclonal antibody (Product # PA5-120401) using a dilution of 1:100. Blue: DAPI for nuclear staining.