MA1-26180

antibody from Invitrogen Antibodies

Targeting: MYH7B dJ756N5.1, KIAA1512, MHC14, MYH14

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-26180

Provider

Invitrogen Antibodies

Product name

Cardiac Myosin Heavy Chain Monoclonal Antibody (3-48)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

This antibody is myosin alpha and beta heavy chain specific. Affinity constants: Human ventricular myosin heavy chains - 3.33 x 108M-1; Human atrial myosin heavy chains - 1.48 x 108M-1; Human skeletal muscle myosin - 1.06 x 108M-1; Canine cardiac myosin heavy chains - 2.06 x 108M-1. This antibody cross-reacts with heavy chains of human myosin isolated from slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide. It does not react with human platelets, smooth muscle myosin heavy chains, myosin light chains, myosin fragments circulating in blood of patients with myocardial infarction, nor does it react with any other human tissue antigens or blood cells. Recommended positive control: Slow skeletal muscle, fast skeletal muscle, eye muscle.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Porcine, Rabbit

Host

Mouse

Isotype

IgG

Antibody clone number

Mar-48

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 HQQR improves Ang II-induced cardiomyocyte hypertrophy. (A) Immunofluorescence experiments were performed using anti-alpha-actinin antibodies to analyze cardiomyocyte hypertrophy (magnification, x200; scale bar, 50 um). (B) mRNA expression levels of the myocardial hypertrophy markers ANP, BNP and beta-MHC were examined using reverse transcription-quantitative PCR. (C) Protein expression levels of the myocardial hypertrophy markers ANP, BNP and beta-MHC were examined using western blotting. ** P