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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PAB7903 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7903, RRID:AB_1716640
- Product name
- TUBB3 (phospho S172) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of TUBB3.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Microtubule regulation in mitosis: tubulin phosphorylation by the cyclin-dependent kinase Cdk1.
Post-translational modifications regulate microtubule function.
Expression of unphosphorylated class III beta-tubulin isotype in neuroepithelial cells demonstrates neuroblast commitment and differentiation.
Fourest-Lieuvin A, Peris L, Gache V, Garcia-Saez I, Juillan-Binard C, Lantez V, Job D
Molecular biology of the cell 2006 Mar;17(3):1041-50
Molecular biology of the cell 2006 Mar;17(3):1041-50
Post-translational modifications regulate microtubule function.
Westermann S, Weber K
Nature reviews. Molecular cell biology 2003 Dec;4(12):938-47
Nature reviews. Molecular cell biology 2003 Dec;4(12):938-47
Expression of unphosphorylated class III beta-tubulin isotype in neuroepithelial cells demonstrates neuroblast commitment and differentiation.
Fanarraga ML, Avila J, Zabala JC
The European journal of neuroscience 1999 Feb;11(2):517-27
The European journal of neuroscience 1999 Feb;11(2):517-27
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of purified human brain tubulin untreated (lane 1) or treated with ERK2 kinase to phosphorylate Ser-172 (lane 2). The blot was probed with TUBB3 (phospho S172) polyclonal antibody (Cat # PAB7903).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunocytochemical labeling in C2C12 cells using TUBB3 (phospho S172) polyclonal antibody (Cat # PAB7903) (A). The specificity of the binding for the latter antibody was demonstrated by using the antibody in the presence of TUBB3 (phospho S172) polyclonal antibody (Cat # PAB7903) (B).
- Validation comment
- Immunofluorescence
