AMAb91395

antibody from Atlas Antibodies

Targeting: TUBB3 beta-4, CFEOM3, CFEOM3A, FEOM3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AMAb91395

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb91395, RRID:AB_2716671

Product name

Anti-TUBB3

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

YEDDEEESEAQGPK


Isotype

IgG

Antibody clone number

CL5814

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line SH-SY5Y.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cerebral cortex tissue.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of WM-115 cells using the Anti-TUBB3 monoclonal antibody, showing specific staining on microtubules in green. Nuclear probe is visualized in blue.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of SH-SY5Y cells using the Anti-TUBB3 monoclonal antibody, showing specific staining on microtubules in green. Nuclear probe is visualized in blue.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of A549 cells using the Anti-TUBB3 monoclonal antibody, showing specific staining on microtubules in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of rat cerebral cortex shows strong positivity in neuronal cell bodies and in neuropil.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil as well as in neurons.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum shows strong positivity in both neuronal cell bodies and neuropil.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows strong positivity in the peripheral nerves.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).