STJ11101457

antibody from St John's Laboratory

Targeting: TUBB3 beta-4, CFEOM3, CFEOM3A, FEOM3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

STJ11101457

Provider

St John's Laboratory

Product name

Anti-TUBB3 antibody [ARC0456] (STJ11101457)

Antibody type

Monoclonal

Description

Rabbit monoclonal antibody anti-Beta III-Tubulin is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

NA


Epitope

NA

Isotype

IgG

Antibody clone number

NA

Vial size

NA

Concentration

NA

Storage

Store in a freezer at-20°C and avoid freeze-thaw cycles.

Handling

NA

Western blot

Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using Beta III-Tubulin/Beta 3-Tubulin antibody (STJ11101457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

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Immunohistochemistry

Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Immunofluorescence analysis of SH-SY5Y cells using Beta III-Tubulin/Beta 3-Tubulin rabbit monoclonal antibody (STJ11101457) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

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Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Immunofluorescence analysis of mouse brain using Beta III-Tubulin/Beta 3-Tubulin rabbit monoclonal antibody (STJ11101457) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

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Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Immunofluorescence analysis of rat brain using Beta III-Tubulin/Beta 3-Tubulin rabbit monoclonal antibody (STJ11101457) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

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Chromatin Immunoprecipitation

Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Immunoprecipitation analysis of 300ug extracts of SH-SY5Y cells using 3ug Beta III-Tubulin antibody (STJ11101457). Western blot was performed from the immunoprecipitate using Beta III-Tubulin antibody (STJ11101457) at a dilition of 1:3000.

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

Show more