Ab091154

antibody from Aladdin Scientific

Targeting: TUBB3 beta-4, CFEOM3, CFEOM3A, FEOM3

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab091154

Provider

Aladdin Scientific

Product name

Recombinant beta III Tubulin Antibody

Antibody type

Recombinant

Description

Rabbit anti Human Beta III Tubulin Antibody, Recombinant (RR607), could be used for WB, Flow and so on.Application:WB: 1/2000 - 1/5000 ÊÊÊÊÊÊFlow: 1/200 - 1/1000Protein FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.Post-translationalSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

RR607

Vial size

100_l,10_l,1ml,50_l

Concentration

0,088 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant beta III Tubulin Antibody (Ab091154) - Western Blot All lanes: Recombinant beta III Tubulin Antibody (Ab091154) at 1/5000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 50 kDa Observed band size: 51 kDa Exposure time: 6.3 s

Flow cytometry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant beta III Tubulin Antibody (Ab091154) - Flow Cytometry Overlay histogram showing HeLa cells stained with Recombinant beta III Tubulin Antibody (Ab091154) (gold). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant beta III Tubulin Antibody (Ab091154) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Red) was used as a control.