PA5-55537

antibody from Invitrogen Antibodies

Targeting: CCDC170 bA282P11.1, C6orf97, FLJ23305

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-55537

Provider

Invitrogen Antibodies

Product name

C6orf97 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: ETINVHEMEA KASRETIMRL ASEVNREQKK AASCTEEKEK LNQDLLSAVE AKEALEREVK IFQERLLAGQ QVWDASKQEV SLLKKSSSEL EKSLKASQDA VT Highest antigen sequence identity to the following orthologs: Mouse - 71%, Rat - 68%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Three functional polymorphisms in CCDC170 were associated with osteoporosis phenotype.

Liu X, Li YG, Tan F, Liu J, Yi R, Zhao X
Biology open 2021 Apr 15;10(4)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2. MiRNAs differentially regulated the allele variants of GWAS lead SNPs. (A-E) Schematic diagram of the miR-153-3p, miR-374b-3p, miR-4274, miR-572 and miR-2964a-5p binding sites on CCDC170 . (F) The overexpression of miR-153-3p significantly suppressed rs6932603-T allele levels in U2OS cells and 293T cells. (G,H) The overexpression of miR-374b-3p and miR-4274 significantly suppressed rs3757322-T allele levels in U2OS cells and 293T cells. (I,J) The overexpression of miR-572 and miR-2964a-5p significantly suppressed rs3748806-G allele levels in U2OS cells and 293T cells. (K) Knockdown of miR-153-3p significantly upregulated rs6932603-T allele levels in U2OS cells and 293T cells. (L,M) Knockdown of miR-374b-3p and miR-4274 significantly upregulated rs3757322-T allele levels in U2OS cells and 293T cells. (N,O) Knockdown of miR-572 and miR-2964a-5p significantly upregulated rs3748806-G allele levels in U2OS cells and 293T cells. (P) Overexpression of these five miRNAs can reduce the expression of CCDC170 at the protein level, while inhibition of this five miRNAs can increase the expression of CCDC170 . The data are from three independent transfection experiments, with assays performed in triplicate ( n =6). Firefly luciferase activity was normalized to Renilla luciferase activity. Error bars show the standard deviation for six technical replicates of a representative experiment. P -values were calculated using a two-tailed Student''s t -test. ** P