Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP16230PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP16230PU-N, RRID:AB_1929089
- Product name
- anti IFT74 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Peptide from the C Terminus of the protein sequence according to NP_001092692.1; NP_001092693.1; NP_079379.2; AAK77221.1.
- Reactivity
- Human, Rat
- Host
- Goat
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- CMG1 antibody staining (AP16230PU-N at 0.3µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP16230PU-N (2.5mg/ml) staining of A) primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor.)
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP16230PU-N (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.