18266-1-AP

antibody from Invitrogen Antibodies

Targeting: PNN memA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

18266-1-AP

Provider

Invitrogen Antibodies

Product name

Pinin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: EKQQDSQPE EVMDVLEMVE NVKHVIADQE VMETNRVESV EPSENEASKE LEPEMEFEIE PDKECKSLSP GKENVSALDM EKESEEKEEK ESEPQPEPVA QPQPQSQPQL QLQSQSQPVL QSQPPSQPED LSLAVLQPTP QVTQEQGHLL PERKDFPVES VKLTEVPVEP VLTVHPESKS KTKTRSRSRG RARNKTSKSR SRSSSSSSSS SSSTSSSSGS SSSSGSSSSR SSSSSSSSTS GSSSRDSSSS TSSSSESRSR SRGRGHNRDR KHRRSVDRKR RDTSGLERSH KSSKGGSSRD TKGSKDKNSR SDRKRSISES SRSGKRSSRS ERDRKSDRKD KRR (376-717 aa encoded by BC062602)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.26 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 18266-1-AP (Pinin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HT-1080 cells were subjected to SDS PAGE followed by western blot with 18266-1-AP (Pinin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 18266-1-AP (Pinin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 18266-1-AP ( Pinin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Pinin antibody (Product # 18266-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PNN antibody 18266-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 18266-1-AP (Pinin antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 18266-1-AP (Pinin antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Pinin (IP:18266-1-AP, 4ug; Detection:18266-1-AP 1:300) with HeLa cells lysate 520ug.