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LearnR31638
antibody from NSJ Bioreagents
Targeting: ITPR1
ACV, Insp3r1, IP3R1, PPP1R94, SCA15, SCA16, SCA29
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Flow cytometry [1]
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Validation data
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- Product number
- R31638 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- IP3R Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific IP3R antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the IP3R antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of IP3R antibody and Lane 1: rat brain; 2: (r) liver; 3: human HeLa; 4: (h) HEPG2. Expected/observed size ~314KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of IP3R antibody and recombinant human protein (0.5ng)
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human SHG-44 (glioma), 2) rat brain and 3) mouse brain lysate with IP3R antibody at 1ug/ml. Predicted molecular weight ~314 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human SHG-44 (glioma), 2) rat brain and 3) mouse brain lysate with IP3R antibody at 1ug/ml. Predicted molecular weight ~314 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) rat brain and 3) mouse brain lysate with IP3R antibody at 1ug/ml. Predicted molecular weight ~314 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with IP3R antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: IP3R antibody testing of human lung cancer tissue. HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: IP3R antibody testing of mouse brain tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P testing of rat brain tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: IP3R antibody testing of mouse brain tissue. HIER: steam section in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P testing of rat brain tissue. HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-87 MG cells with IP3R antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IP3R antibody.
