PA5-85753

antibody from Invitrogen Antibodies

Targeting: ITPR1 ACV, Insp3r1, IP3R1, PPP1R94, SCA15, SCA16, SCA29

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-85753

Provider

Invitrogen Antibodies

Product name

IP3 Receptor 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Keep as concentrated solution.

Concentration

1.04 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IP3 Receptor 1 in various tissue extracts using IP3 Receptor 1 polyclonal antibody (Product # PA5-85753) using 50 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using IP3 Receptor 1 Polyclonal Antibody (Product # PA5-85753). Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor 1 Polyclonal Antibody (Product # PA5-85753) diluted at 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP3 Receptor 1 Polyclonal Antibody detects IP3 Receptor I protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Itpr1 protein stained by IP3 Receptor 1 Polyclonal Antibody (Product # PA5-85753) diluted at 1:250. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of IP3 Receptor 1 was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: IP3 Receptor 1 Polyclonal Antibody (Product # PA5 85753) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP3 Receptor 1 Polyclonal Antibody detects IP3 Receptor I protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: IP3 Receptor I protein stained by IP3 Receptor 1 Polyclonal Antibody (Product # PA5-85753) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .