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antibody from Invitrogen Antibodies
Targeting: ITPR1
ACV, Insp3r1, IP3R1, PPP1R94, SCA15, SCA16, SCA29
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-85753 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IP3 Receptor 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Chicken (100%). Positive Control: mouse brain, rat brain. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.04 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IP3 Receptor 1 in various tissue extracts using IP3 Receptor 1 polyclonal antibody (Product # PA5-85753) using 50 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IP3 Receptor 1 Polyclonal Antibody (Product # PA5-85753). Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor 1 Polyclonal Antibody (Product # PA5-85753) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP3 Receptor 1 Polyclonal Antibody detects IP3 Receptor I protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Itpr1 protein stained by IP3 Receptor 1 Polyclonal Antibody (Product # PA5-85753) diluted at 1:250. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of IP3 Receptor 1 was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: IP3 Receptor 1 Polyclonal Antibody (Product # PA5 85753) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP3 Receptor 1 Polyclonal Antibody detects IP3 Receptor I protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: IP3 Receptor I protein stained by IP3 Receptor 1 Polyclonal Antibody (Product # PA5-85753) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
