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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- RQ4928 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- DOK7 Antibody / Docking protein 7
- Antibody type
- Polyclonal
- Description
- This highly specific DOK7 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the DOK7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human PC-3, 3) human HL-60, 4) rat skeletal muscle, 5) rat heart, 6) mouse skeletal muscle and 7) mouse heart lysate with DOK7 antibody at 0.5ug/ml. Predicted molecular weight ~53 kDa, observed here at ~65 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) MCF7 and 2) RT4 cell lysate with DOK7 antibody. Predicted molecular weight ~53 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat skeletal muscle, 2) rat brain, 3) rat heart, 4) rat L6, 5) mouse skeletal muscle, 6) mouse brain, 7) mouse heart and 8) mouse C2C12 cell lysate with DOK7 antibody. Predicted molecular weight ~53 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of FFPE human A431 cells with DOK7 antibody at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human skeletal muscle tissue with DOK7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat heart tissue with DOK7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human RT4 cells with DOK7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DOK7 antibody.
