HPA051870

antibody from Atlas Antibodies

Targeting: TMEM119 OBIF

Provider product page for HPA051870

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [48]
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Validations
Immunohistochemistry [1]

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Product number: HPA051870 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA051870, RRID:AB_2681645
Product name: Anti-TMEM119
Antibody type: Polyclonal
Description: Polyclonal Antibody against Human TMEM119, Gene description: transmembrane protein 119, Validated applications: IHC, Uniprot ID: Q4V9L6, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100 µl
Concentration: 0.1 mg/ml
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Handling: The antibody solution should be gently mixed before use.

TMEM119 protein structure - HPA051870 shown in red.

Submitted references Human Microglia–Like Cells Differentiated from Monocytes with GM-CSF and IL-34 Show Phagocytosis of α-Synuclein Aggregates and C/EBPβ-Dependent Proinflammatory Activation

Llaves-López A, Micoli E, Belmonte-Mateos C, Aguilar G, Alba C, Marsal A, Pulido-Salgado M, Rabaneda-Lombarte N, Solà C, Serratosa J, Vidal-Taboada J, Saura J
Molecular Neurobiology 2024;62(1):756-772

A glia-enriched stem cell 3D model of the human brain mimics the glial-immune neurodegenerative phenotypes of multiple sclerosis

Fagiani F, Pedrini E, Taverna S, Brambilla E, Murtaj V, Podini P, Ruffini F, Butti E, Braccia C, Andolfo A, Magliozzi R, Smirnova L, Kuhlmann T, Quattrini A, Calabresi P, Reich D, Martino G, Panina-Bordignon P, Absinta M
Cell Reports Medicine 2024;5(8):101680

Myeloid cell-derived creatine in the hypoxic niche promotes glioblastoma growth

Rashidi A, Billingham L, Zolp A, Chia T, Silvers C, Katz J, Park C, Delay S, Boland L, Geng Y, Markwell S, Dmello C, Arrieta V, Zilinger K, Jacob I, Lopez-Rosas A, Hou D, Castro B, Steffens A, McCortney K, Walshon J, Flowers M, Lin H, Wang H, Zhao J, Sonabend A, Zhang P, Ahmed A, Brat D, Heiland D, Lee-Chang C, Lesniak M, Chandel N, Miska J
Cell Metabolism 2024;36(1):62-77.e8

Bone marrow-derived myeloid cells transiently colonize the brain during postnatal development and interact with glutamatergic synapses

Carrier M, Robert M, St-Pierre M, Ibáñez F, Gonçalves de Andrade E, Laroche A, Picard K, Vecchiarelli H, Savage J, Boilard É, Desjardins M, Tremblay M
iScience 2024;27(7):110037

CHIT1 at diagnosis predicts faster disability progression and reflects early microglial activation in multiple sclerosis

Beliën J, Swinnen S, D’hondt R, Verdú de Juan L, Dedoncker N, Matthys P, Bauer J, Vens C, Moylett S, Dubois B
Nature Communications 2024;15(1)

Ultrasound-mediated delivery of doxorubicin to the brain results in immune modulation and improved responses to PD-1 blockade in gliomas

Arrieta V, Gould A, Kim K, Habashy K, Dmello C, Vázquez-Cervantes G, Palacín-Aliana I, McManus G, Amidei C, Gomez C, Dhiantravan S, Chen L, Zhang D, Saganty R, Cholak M, Pandey S, McCord M, McCortney K, Castro B, Ward R, Muzzio M, Bouchoux G, Desseaux C, Canney M, Carpentier A, Zhang B, Miska J, Lesniak M, Horbinski C, Lukas R, Stupp R, Lee-Chang C, Sonabend A
Nature Communications 2024;15(1)

Landscape of brain myeloid cell transcriptome along the spatiotemporal progression of Alzheimer’s disease reveals distinct sequential responses to Aβ and tau

Wachter A, Woodbury M, Lombardo S, Abdourahman A, Wuest C, McGlame E, Pastika T, Tamm J, Romanul N, Yanamandra K, Bennett R, Lin G, Kwon T, Liao F, Klein C, Grinberg Y, Jaisa-aad M, Li H, Frosch M, Kummer M, Das S, Dellovade T, Karran E, Langlois X, Ried J, Serrano-Pozo A, Talanian R, Biber K, Hyman B
Acta Neuropathologica 2024;147(1)

The CXCL16-CXCR6 axis in glioblastoma modulates T-cell activity in a spatiotemporal context

Chia T, Billingham L, Boland L, Katz J, Arrieta V, Shireman J, Rosas A, DeLay S, Zillinger K, Geng Y, Kruger J, Silvers C, Wang H, Vazquez Cervantes G, Hou D, Wang S, Wan H, Sonabend A, Zhang P, Lee-Chang C, Miska J
Frontiers in Immunology 2024;14

Human-induced pluripotent stem cell-derived microglia integrate into mouse retina and recapitulate features of endogenous microglia

Ma W, Zhao L, Xu B, Fariss R, Redmond T, Zou J, Wong W, Li W
eLife 2024;12

Huuki-Myers L, Montgomery K, Kwon S, Cinquemani S, Eagles N, Gonzalez-Padilla D, Maden S, Kleinman J, Hyde T, Hicks S, Maynard K, Collado-Torres L
2024

Thompson J, Nelson E, Tippani M, Ramnauth A, Divecha H, Miller R, Eagles N, Pattie E, Kwon S, Bach S, Kaipa U, Yao J, Hou C, Kleinman J, Collado-Torres L, Han S, Maynard K, Hyde T, Martinowich K, Page S, Hicks S
2024

ATP1A3 as a target for isolating neuron-specific extracellular vesicles from human brain and biofluids

You Y, Zhang Z, Sultana N, Ericsson M, Martens Y, Sun M, Kanekiyo T, Ikezu S, Shaffer S, Ikezu T
Science Advances 2023;9(37)

A potential protective role of the nuclear receptor-related factor 1 (Nurr1) in multiple sclerosis motor cortex: a neuropathological study

Pansieri J, Pisa M, Yates R, Esiri M, DeLuca G
Brain Communications 2023;5(2)

Tau activation of microglial cGAS–IFN reduces MEF2C-mediated cognitive resilience

Udeochu J, Amin S, Huang Y, Fan L, Torres E, Carling G, Liu B, McGurran H, Coronas-Samano G, Kauwe G, Mousa G, Wong M, Ye P, Nagiri R, Lo I, Holtzman J, Corona C, Yarahmady A, Gill M, Raju R, Mok S, Gong S, Luo W, Zhao M, Tracy T, Ratan R, Tsai L, Sinha S, Gan L
Nature Neuroscience 2023;26(5):737-750

GBMdeconvoluteR accurately infers proportions of neoplastic and immune cell populations from bulk glioblastoma transcriptomics data

Ajaib S, Lodha D, Pollock S, Hemmings G, Finetti M, Gusnanto A, Chakrabarty A, Ismail A, Wilson E, Varn F, Hunter B, Filby A, Brockman A, McDonald D, Verhaak R, Ihrie R, Stead L
Neuro-Oncology 2023;25(7):1236-1248

Cerebellar presence of immune cells in patients with neuro-coeliac disease

Rouvroye M, Bontkes H, Bol J, Lissenberg-Witte B, Byrnes V, Bennani F, Jordanova E, Wilhelmus M, Mulder C, van der Valk P, Rozemuller A, Bouma G, Van Dam A
Acta Neuropathologica Communications 2023;11(1)

The localization of molecularly distinct microglia populations to Alzheimer's disease pathologies using QUIVER

Shahidehpour R, Nelson A, Sanders L, Embry C, Nelson P, Bachstetter A
Acta Neuropathologica Communications 2023;11(1)

Que Z, Olivero-Acosta M, Chen I, Zhang J, Wettschurack K, Wu J, Xiao T, Otterbacher C, Wang M, Harlow H, Cui N, Chen X, Deming B, Halurkar M, Zhao Y, Rochet J, Xu R, Brewster A, Wu L, Yuan C, Skarnes W, Yang Y
2023

Apolipoprotein E4 impairs the response of neurodegenerative retinal microglia and prevents neuronal loss in glaucoma

Margeta M, Yin Z, Madore C, Pitts K, Letcher S, Tang J, Jiang S, Gauthier C, Silveira S, Schroeder C, Lad E, Proia A, Tanzi R, Holtzman D, Krasemann S, Chen D, Butovsky O
Immunity 2022;55(9):1627-1644.e7

A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation

Bartalska K, Hübschmann V, Korkut-Demirbaş M, Cubero R, Venturino A, Rössler K, Czech T, Siegert S
iScience 2022;25(7):104580

Immunopathology of the optic nerve in multiple sclerosis

Fernández Blanco L, Marzin M, Leistra A, van der Valk P, Nutma E, Amor S
Clinical and Experimental Immunology 2022;209(2):236-246

Dominant-acting CSF1R variants cause microglial depletion and altered astrocytic phenotype in zebrafish and adult-onset leukodystrophy

Berdowski W, van der Linde H, Breur M, Oosterhof N, Beerepoot S, Sanderson L, Wijnands L, de Jong P, Tsai-Meu-Chong E, de Valk W, de Witte M, van IJcken W, Demmers J, van der Knaap M, Bugiani M, Wolf N, van Ham T
Acta Neuropathologica 2022;144(2):211-239

Advances in Visualizing Microglial Cells in Human Central Nervous System Tissue

Uff C, Patel K, Yeung C, Yip P
Biomolecules 2022;12(5):603

Molecular Signature of Neuroinflammation Induced in Cytokine-Stimulated Human Cortical Spheroids

De Kleijn K, Straasheijm K, Zuure W, Martens G
Biomedicines 2022;10(5):1025

Changes in glial cell phenotypes precede overt neurofibrillary tangle formation, correlate with markers of cortical cell damage, and predict cognitive status of individuals at Braak III-IV stages

Taddei R, Sanchez-Mico M, Bonnar O, Connors T, Gaona A, Denbow D, Frosch M, Gómez-Isla T
Acta Neuropathologica Communications 2022;10(1)

Hou D, Castro B, Dapash M, Zolp A, Katz J, Arrieta V, Biermann J, Melms J, Kueckelhaus J, Benotmane J, Youngblood M, Rashidi A, Billingham L, Dmello C, Vazquez-Cervantes G, Lopez-Rosas A, Han Y, Patel R, Chia T, Sun L, Prins R, Izar B, Heiland D, Zhang P, Sonabend A, Miska J, Lesniak M, Zhao J, Lee-Chang C
2022

Activated microglia do not increase 18 kDa translocator protein (TSPO) expression in the multiple sclerosis brain

Nutma E, Gebro E, Marzin M, van der Valk P, Matthews P, Owen D, Amor S
Glia 2021;69(10):2447-2458

MicroRNA‐34a activation in tuberous sclerosis complex during early brain development may lead to impaired corticogenesis

Korotkov A, Sim N, Luinenburg M, Anink J, van Scheppingen J, Zimmer T, Bongaarts A, Broekaart D, Mijnsbergen C, Jansen F, Van Hecke W, Spliet W, van Rijen P, Feucht M, Hainfellner J, Kršek P, Zamecnik J, Crino P, Kotulska K, Lagae L, Jansen A, Kwiatkowski D, Jozwiak S, Curatolo P, Mühlebner A, Lee J, Mills J, van Vliet E, Aronica E
Neuropathology and Applied Neurobiology 2021;47(6):796-811

The influence of HLA‐DRB1*15 on the relationship between microglia and neurons in multiple sclerosis normal appearing cortical grey matter

Yates R, Pansieri J, Li Q, Bell J, Yee S, Palace J, Esiri M, DeLuca G
Brain Pathology 2021;32(4)

Meningeal inflammation in multiple sclerosis induces phenotypic changes in cortical microglia that differentially associate with neurodegeneration

van Olst L, Rodriguez-Mogeda C, Picon C, Kiljan S, James R, Kamermans A, van der Pol S, Knoop L, Michailidou I, Drost E, Franssen M, Schenk G, Geurts J, Amor S, Mazarakis N, van Horssen J, de Vries H, Reynolds R, Witte M
Acta Neuropathologica 2021;141(6):881-899

A loss of mature microglial markers without immune activation in schizophrenia.

Snijders GJLJ, van Zuiden W, Sneeboer MAM, Berdenis van Berlekom A, van der Geest AT, Schnieder T, MacIntyre DJ, Hol EM, Kahn RS, de Witte LD
Glia 2021 May;69(5):1251-1267

VISTA regulates microglia homeostasis and myelin phagocytosis, and is associated with MS lesion pathology.

Borggrewe M, Kooistra SM, Wesseling EM, Gierschek FL, Brummer ML, Nowak EC, Medeiros-Furquim T, Otto TA, Lee SW, Noelle RJ, Eggen BJL, Laman JD
Acta neuropathologica communications 2021 May 18;9(1):91

Poly-ADP-ribosylation drives loss of protein homeostasis in ATM and Mre11 deficiency.

Lee JH, Ryu SW, Ender NA, Paull TT
Molecular cell 2021 Apr 1;81(7):1515-1533.e5

Microglia promote glioblastoma via mTOR‐mediated immunosuppression of the tumour microenvironment

Dumas A, Pomella N, Rosser G, Guglielmi L, Vinel C, Millner T, Rees J, Aley N, Sheer D, Wei J, Marisetty A, Heimberger A, Bowman R, Brandner S, Joyce J, Marino S
The EMBO Journal 2020;39(15)

Distinct microglial and macrophage distribution patterns in the concentric and lamellar lesions in Baló's disease and neuromyelitis optica spectrum disorders

Hayashida S, Masaki K, Suzuki S, Yamasaki R, Watanabe M, Koyama S, Isobe N, Matsushita T, Takahashi K, Tabira T, Iwaki T, Kira J
Brain Pathology 2020;30(6):1144-1157

Increased expression of miR142 and miR155 in glial and immune cells after traumatic brain injury may contribute to neuroinflammation via astrocyte activation

Korotkov A, Puhakka N, Gupta S, Vuokila N, Broekaart D, Anink J, Heiskanen M, Karttunen J, van Scheppingen J, Huitinga I, Mills J, van Vliet E, Pitkänen A, Aronica E
Brain Pathology 2020;30(5):897-912

Microglia express TMEM119 in the brains of Nasu-Hakola disease

Satoh J, Kino Y, Yanaizu M, Ishida T, Saito Y
Intractable & Rare Diseases Research 2019;8(4):260-265

Multiplexed imaging of immune cells in staged multiple sclerosis lesions by mass cytometry

Ramaglia V, Sheikh-Mohamed S, Legg K, Park C, Rojas O, Zandee S, Fu F, Ornatsky O, Swanson E, Pitt D, Prat A, McKee T, Gommerman J
eLife 2019;8

Microglia damage precedes major myelin breakdown in X‐linked adrenoleukodystrophy and metachromatic leukodystrophy

Bergner C, van der Meer F, Winkler A, Wrzos C, Türkmen M, Valizada E, Fitzner D, Hametner S, Hartmann C, Pfeifenbring S, Stoltenburg‐Didinger G, Brück W, Nessler S, Stadelmann C
Glia 2019;67(6):1196-1209

Single-Cell RNA Sequencing of Microglia throughout the Mouse Lifespan and in the Injured Brain Reveals Complex Cell-State Changes

Hammond T, Dufort C, Dissing-Olesen L, Giera S, Young A, Wysoker A, Walker A, Gergits F, Segel M, Nemesh J, Marsh S, Saunders A, Macosko E, Ginhoux F, Chen J, Franklin R, Piao X, McCarroll S, Stevens B
Immunity 2019;50(1):253-271.e6

A quantitative neuropathological assessment of translocator protein expression in multiple sclerosis

Nutma E, Stephenson J, Gorter R, de Bruin J, Boucherie D, Donat C, Breur M, van der Valk P, Matthews P, Owen D, Amor S
Brain 2019;142(11):3440-3455

Regulation of microglial TMEM119 and P2RY12 immunoreactivity in multiple sclerosis white and grey matter lesions is dependent on their inflammatory environment.

van Wageningen TA, Vlaar E, Kooij G, Jongenelen CAM, Geurts JJG, van Dam AM
Acta neuropathologica communications 2019 Dec 11;7(1):206

TLR-stimulated IRAKM activates caspase-8 inflammasome in microglia and promotes neuroinflammation

Zhang C, Jiang M, Zhou H, Liu W, Wang C, Kang Z, Han B, Zhang Q, Chen X, Xiao J, Fisher A, Kaiser W, Murayama M, Iwakura Y, Gao J, Carman J, Dongre A, Dubyak G, Abbott D, Shi F, Ransohoff R, Li X
Journal of Clinical Investigation 2018;128(12):5399-5412

TMEM119 silencing inhibits cell viability and causes the apoptosis of gastric cancer SGC-7901 cells

Zheng P, Wang W, Ji M, Zhu Q, Feng Y, Zhou F, He Q
Oncology Letters 2018

The TREM2-APOE Pathway Drives the Transcriptional Phenotype of Dysfunctional Microglia in Neurodegenerative Diseases

Krasemann S, Madore C, Cialic R, Baufeld C, Calcagno N, El Fatimy R, Beckers L, O’Loughlin E, Xu Y, Fanek Z, Greco D, Smith S, Tweet G, Humulock Z, Zrzavy T, Conde-Sanroman P, Gacias M, Weng Z, Chen H, Tjon E, Mazaheri F, Hartmann K, Madi A, Ulrich J, Glatzel M, Worthmann A, Heeren J, Budnik B, Lemere C, Ikezu T, Heppner F, Litvak V, Holtzman D, Lassmann H, Weiner H, Ochando J, Haass C, Butovsky O
Immunity 2017;47(3):566-581.e9

Dominant role of microglial and macrophage innate immune responses in human ischemic infarcts

Zrzavy T, Machado‐Santos J, Christine S, Baumgartner C, Weiner H, Butovsky O, Lassmann H
Brain Pathology 2017;28(6):791-805

Transcriptomic analysis of purified human cortical microglia reveals age-associated changes

Galatro T, Holtman I, Lerario A, Vainchtein I, Brouwer N, Sola P, Veras M, Pereira T, Leite R, Möller T, Wes P, Sogayar M, Laman J, den Dunnen W, Pasqualucci C, Oba-Shinjo S, Boddeke E, Marie S, Eggen B
Nature Neuroscience 2017;20(8):1162-1171

Loss of ‘homeostatic’ microglia and patterns of their activation in active multiple sclerosis

Zrzavy T, Hametner S, Wimmer I, Butovsky O, Weiner H, Lassmann H
Brain 2017;140(7):1900-1913

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human lymph node and liver tissues using HPA051870 antibody. Corresponding TMEM119 RNA-seq data are presented for the same tissues.
Sample type: Human
Protocol: Protocol
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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