Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 44-876G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-CD61 (Tyr773) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Src Family Kinases Modulate the Loss of Endothelial Barrier Function in Response to TNF-α: Crosstalk with p38 Signaling.
Antithrombotic effects of targeting alphaIIbbeta3 signaling in platelets.
Eph kinases and ephrins support thrombus growth and stability by regulating integrin outside-in signaling in platelets.
Focal complex formation in adult cardiomyocytes is accompanied by the activation of beta3 integrin and c-Src.
Focal complex formation in adult cardiomyocytes is accompanied by the activation of beta3 integrin and c-Src.
Adam AP, Lowery AM, Martino N, Alsaffar H, Vincent PA
PloS one 2016;11(9):e0161975
PloS one 2016;11(9):e0161975
Antithrombotic effects of targeting alphaIIbbeta3 signaling in platelets.
Ablooglu AJ, Kang J, Petrich BG, Ginsberg MH, Shattil SJ
Blood 2009 Apr 9;113(15):3585-92
Blood 2009 Apr 9;113(15):3585-92
Eph kinases and ephrins support thrombus growth and stability by regulating integrin outside-in signaling in platelets.
Prévost N, Woulfe DS, Jiang H, Stalker TJ, Marchese P, Ruggeri ZM, Brass LF
Proceedings of the National Academy of Sciences of the United States of America 2005 Jul 12;102(28):9820-5
Proceedings of the National Academy of Sciences of the United States of America 2005 Jul 12;102(28):9820-5
Focal complex formation in adult cardiomyocytes is accompanied by the activation of beta3 integrin and c-Src.
Willey CD, Balasubramanian S, Rodríguez Rosas MC, Ross RS, Kuppuswamy D
Journal of molecular and cellular cardiology 2003 Jun;35(6):671-83
Journal of molecular and cellular cardiology 2003 Jun;35(6):671-83
Focal complex formation in adult cardiomyocytes is accompanied by the activation of beta3 integrin and c-Src.
Willey CD, Balasubramanian S, Rodríguez Rosas MC, Ross RS, Kuppuswamy D
Journal of molecular and cellular cardiology 2003 Jun;35(6):671-83
Journal of molecular and cellular cardiology 2003 Jun;35(6):671-83
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Melanoma cells transfected with human Integrin av and wild-type (WT) or mutant (Y773F) human Integrin beta3 were analyzed. Integrin beta3 was immunoprecipitated then incubated with Integrin beta3 (pY773) polyclonal antibody along with lysates corresponding to non-phosphorylated Integrin beta3 (1), phosphorylated WT Integrin beta3 (2), or phosphorylated Y773F mutant Integrin beta3 (3). Elimination of signal in site-directed mutant demonstrates antibody specificity.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Melanoma cells transfected with human Integrin av and wild-type (WT) or mutant (Y773F) human Integrin beta3 were analyzed. Integrin beta3 was immunoprecipitated then incubated with Integrin beta3 (pY773) polyclonal antibody along with lysates corresponding to non-phosphorylated Integrin beta3 (1), phosphorylated WT Integrin beta3 (2), or phosphorylated Y773F mutant Integrin beta3 (3). Elimination of signal in site-directed mutant demonstrates antibody specificity.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Melanoma cells transfected with human Integrin av and wild-type (WT) or mutant (Y773F) human Integrin beta3 were analyzed. Integrin beta3 was immunoprecipitated then incubated with Integrin beta3 (pY773) polyclonal antibody along with lysates corresponding to non-phosphorylated Integrin beta3 (1), phosphorylated WT Integrin beta3 (2), or phosphorylated Y773F mutant Integrin beta3 (3). Elimination of signal in site-directed mutant demonstrates antibody specificity.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Phospho integrin beta 3 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Integrin beta-3 pTyr773 Rabbit Polyclonal Antibody (Product # 44-876G) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Phospho-CD61 (Tyr773) was performed using 70% confluent log phase HeLa cells treated with 1mM of Paclitaxel for 20 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-CD61 (Tyr773) Rabbit Polyclonal Antibody(Product # 44-876G) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing focal adhesion and membrane localization. Panel e shows untreated cells with reduced cytoplasmic signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Phospho-Integrin beta-3 pTyr773 was done on U-87 MG cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Phospho-Integrin beta-3 pTyr773 Rabbit Polyclonal Antibody (44876G, red histogram) or with rabbit isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 2 Constitutive activation of SFKs through expression of DN-Csk renders HDMEC susceptible to low doses of TNF-alpha. Cells were seeded at confluence and incubated for 72 hours. Then, growth media was replaced by low serum media and cells were infected with adenovirus to express either LacZ (control) or DN-Csk. After another 16 h, cells were treated with low-dose TNF-alpha. A , Western blot analysis demonstrating the increased tyrosine phosphorylation at multiple SFK substrates. B , TEER measurements on ECIS electrodes. C , FITC-Albumin permeability assay on Transwell chambers. D , TEER of cells pretreated with either DMSO or PP2 for 30 minutes prior to TNF-alpha addition. Results are representative of at least three independent experiments. Notice that the synergistic effect of DN-Csk expression concurrently with a low-dose TNF-alpha can be prevented by the SFK inhibitor PP2. Data presented as mean+-SEM (each experiment performed in duplicate). Asterisk, p