Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 11-0519-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin alphav/beta3. CD51, an ~120 kDa surface molecule can also non-covalently associate with other beta subunits of the integrin family including beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by melanoma cells, endothelial cells and osteoclasts and at very low levels by platelets. The CD51/CD61 complex mediates adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.
- Conjugate
- Green dye
- Antibody clone number
- 23C6
- Concentration
- 5 µL/Test
Submitted references Effect of Denosumab on the Change of Osteoclast Precursors Compared to Zoledronate Treatment in Postmenopausal Women with Osteoporosis.
High Density Display of an Anti-Angiogenic Peptide on Micelle Surfaces Enhances Their Inhibition of αvβ3 Integrin-Mediated Neovascularization In Vitro.
Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.
An Efficient Bivalent Cyclic RGD-PIK3CB siRNA Conjugate for Specific Targeted Therapy against Glioblastoma In Vitro and In Vivo.
Interferon-γ enhances both the anti-bacterial and the pro-inflammatory response of human mast cells to Staphylococcus aureus.
Insulin-like growth factor 1 increases apical fibronectin in blastocysts to increase blastocyst attachment to endometrial epithelial cells in vitro.
Tumor-targeted in vivo gene silencing via systemic delivery of cRGD-conjugated siRNA.
Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes.
Kong SH, Kim JH, Kim SW, Jeong AJ, Lee SH, Ye SK, Shin CS
Journal of bone metabolism 2022 May;29(2):93-101
Journal of bone metabolism 2022 May;29(2):93-101
High Density Display of an Anti-Angiogenic Peptide on Micelle Surfaces Enhances Their Inhibition of αvβ3 Integrin-Mediated Neovascularization In Vitro.
Nagaraj R, Stack T, Yi S, Mathew B, Shull KR, Scott EA, Mathew MT, Bijukumar DR
Nanomaterials (Basel, Switzerland) 2020 Mar 22;10(3)
Nanomaterials (Basel, Switzerland) 2020 Mar 22;10(3)
Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.
Menon V, Thomas R, Elgueta C, Horl M, Osborn T, Hallett PJ, Bartos M, Isacson O, Pruszak J
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
Stem cells (Dayton, Ohio) 2019 Oct;37(10):1293-1306
An Efficient Bivalent Cyclic RGD-PIK3CB siRNA Conjugate for Specific Targeted Therapy against Glioblastoma In Vitro and In Vivo.
Cen B, Wei Y, Huang W, Teng M, He S, Li J, Wang W, He G, Bai X, Liu X, Yuan Y, Pan X, Ji A
Molecular therapy. Nucleic acids 2018 Dec 7;13:220-232
Molecular therapy. Nucleic acids 2018 Dec 7;13:220-232
Interferon-γ enhances both the anti-bacterial and the pro-inflammatory response of human mast cells to Staphylococcus aureus.
Swindle EJ, Brown JM, Rådinger M, DeLeo FR, Metcalfe DD
Immunology 2015 Nov;146(3):470-85
Immunology 2015 Nov;146(3):470-85
Insulin-like growth factor 1 increases apical fibronectin in blastocysts to increase blastocyst attachment to endometrial epithelial cells in vitro.
Green CJ, Fraser ST, Day ML
Human reproduction (Oxford, England) 2015 Feb;30(2):284-98
Human reproduction (Oxford, England) 2015 Feb;30(2):284-98
Tumor-targeted in vivo gene silencing via systemic delivery of cRGD-conjugated siRNA.
Liu X, Wang W, Samarsky D, Liu L, Xu Q, Zhang W, Zhu G, Wu P, Zuo X, Deng H, Zhang J, Wu Z, Chen X, Zhao L, Qiu Z, Zhang Z, Zeng Q, Yang W, Zhang B, Ji A
Nucleic acids research 2014 Oct;42(18):11805-17
Nucleic acids research 2014 Oct;42(18):11805-17
Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes.
Sekine C, Koyanagi A, Koyama N, Hozumi K, Chiba S, Yagita H
Arthritis research & therapy 2012 Mar 5;14(2):R45
Arthritis research & therapy 2012 Mar 5;14(2):R45
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of the M21 cell line with Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (open histogram) or Anti-Human CD51/CD61 (Integrin alpha/beta 3) FITC (filled histogram). Total viable cells were used for analysis.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Expression of alphavbeta3 by HUVECs under different conditions ( a ) Flow cytometry analysis demonstrated significant expression of integrin alphavbeta3 in overnight starved cells. (i) Side scatter (SSC) vs. forward scatter (FSC) plot used to gate live cells and resulting (ii) histograms from which alphavbeta3 expression was (iii) quantified for different culture conditions. Colors in (ii) match the conditions shown in the x-axis of (iii). Values were expressed as mean +- SD, n = 3. p < 0.005 was considered significant. ( b ) Immunostaining was performed by (i) staining HUVECs with anti-human CD51/CD61 antibody to detect integrin alphavbeta3 (green). Nuclei were stained by DAPI (blue). Integrin alphavbeta3 expression was highest under overnight starved conditions when compared to control and 2 h starvation. (ii) Quantification of the images in (i) obtained using Image J. Value was expressed as mean +- SD, n = 3. p < 0.05 was considered significant.
- Conjugate
- Green dye