Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-0619-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD61 (Integrin beta 3) Monoclonal Antibody (VI-PL2), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The VI-PL2 monoclonal antibody reacts with human CD61, also known as integrin beta 3 and GPIIIa. CD61 is a 90-110 kDa member of the beta integrin family expressed by a wide variety of cells, including leukocytes, platelets, endothelial and smooth muscle cells. CD61 binds non-covalently with the alpha integrins CD41 and CD51, to form the alpha IIb beta3 (CD41/CD61) and alpha v beta3 (CD51/CD61) complexes. These alpha beta heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. The CD41/CD61 integrin has been shown to be involved in platelet aggregation, and binds to fibrinogen, von Willebrand Factor (vWF) and fibronectin. The CD51/CD61 integrin binds to matrix proteins including vitronectin, fibronectin, vWF and fibrinogen, and has been shown to have a strong role in modulating the migration and survival of angiogenic endothelial cells. This VI-PL2 antibody has also been shown to crossreact with canine and several non-human primate species, including baboon, rhesus, and cynomologus monkey. Applications Reported: This VI-PL2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunohistochemical staining, and functional assays. Applications Tested: This VI-PL2 antibody has been tested by flow cytometric analysis of human peripheral blood platelets. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- VI-PL2
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Circulating CD14-CD36+ peripheral blood mononuclear cells constitutively produce interleukin-10.
Protein kinase B/Akt acts via glycogen synthase kinase 3 to regulate recycling of alpha v beta 3 and alpha 5 beta 1 integrins.
VLA-2 (alpha2beta1) integrin promotes rotavirus entry into cells but is not necessary for rotavirus attachment.
Barrett L, Dai C, Gamberg J, Gallant M, Grant M
Journal of leukocyte biology 2007 Jul;82(1):152-60
Journal of leukocyte biology 2007 Jul;82(1):152-60
Protein kinase B/Akt acts via glycogen synthase kinase 3 to regulate recycling of alpha v beta 3 and alpha 5 beta 1 integrins.
Roberts MS, Woods AJ, Dale TC, Van Der Sluijs P, Norman JC
Molecular and cellular biology 2004 Feb;24(4):1505-15
Molecular and cellular biology 2004 Feb;24(4):1505-15
VLA-2 (alpha2beta1) integrin promotes rotavirus entry into cells but is not necessary for rotavirus attachment.
Ciarlet M, Crawford SE, Cheng E, Blutt SE, Rice DA, Bergelson JM, Estes MK
Journal of virology 2002 Feb;76(3):1109-23
Journal of virology 2002 Feb;76(3):1109-23
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood platelets with 0.06 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (left) or 0.06 µg of Anti-Human CD61 (Integrin beta 3) Purified (right) followed by 'F (ab')2 Anti-Mouse IgG PE (Product # 12-4012) followed by Anti-Human CD42b FITC (Product # 11-0429-42).