PA5-85926

antibody from Invitrogen Antibodies

Targeting: ITGB3 CD61, GP3A, GPIIIa

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-85926

Provider

Invitrogen Antibodies

Product name

CD61 (Integrin beta 3) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

C/D box snoRNA SNORD113-6/AF357425 plays a dual role in integrin signalling and arterial fibroblast function via pre-mRNA processing and 2'O-ribose methylation.

van Ingen E, van den Homberg DAL, van der Bent ML, Mei H, Papac-Milicevic N, Kremer V, Boon RA, Quax PHA, Wojta J, Nossent AY
Human molecular genetics 2022 Mar 31;31(7):1051-1066

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD61 (Integrin beta 3) in A431 (1) and rat spleen tissue (2) lysate using a CD61 (Integrin beta 3) Polyclonal antibody (Product # PA5-85926) at a dilution of 1:1,000.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of CD61 (Integrin beta 3) in A431 cells using a CD61 (Integrin beta 3) Polyclonal Antibody (Product # PA5-85926) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of CD61 (Integrin beta 3) in HUVEC cells using a CD61 (Integrin beta 3) Polyclonal Antibody (Product # PA5-85926) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Protein expression in murine and human primary fibroblasts. ( A ) Western blots for JAG1, DUSP7, ITGB3 and MAP2K1, each with GAPDH, of three independent replicates (each band represents a pool of three technical triplicates) in PMFs. ( B ) Western blots for JAG1, DUSP7, ITGB3 and MAP2K1, with GAPDH of three independent replicates (each band represents a pool of three technical triplicates) in HUAFs. ( C - L ) Quantifications of protein band intensities, normalized to GAPDH. Data are represented as mean +- SEM. * * P < 0.01, compared with GM-ctrl.