BS-0342R

antibody from Invitrogen Antibodies

Targeting: ITGB3 CD61, GP3A, GPIIIa

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

BS-0342R

Provider

Invitrogen Antibodies

Product name

Integrin beta 3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Integrin beta 3/CD61 Polyclonal Antibody, Unconjugated (bs-0342R) at 1:200, followed by conjugation to the secondary antibody and DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HL-60 (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin beta 3 Antibody (bs-0342R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human HL-60 cells probed with Integrin beta 3 Polyclonal Antibody, Unconjugated (bs-0342R) (green) at 1:100 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HL-60 (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin beta 3 Antibody (bs-0342R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).