PA5-36552

antibody from Invitrogen Antibodies

Targeting: AQP3 GIL

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36552

Provider

Invitrogen Antibodies

Product name

Aquaporin 3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 32 kDa. Purity is >95% by SDS-PAGE.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Mitochondrial dysfunction promotes aquaporin expression that controls hydrogen peroxide permeability and ferroptosis.

Takashi Y, Tomita K, Kuwahara Y, Roudkenar MH, Roushandeh AM, Igarashi K, Nagasawa T, Nishitani Y, Sato T
Free radical biology & medicine 2020 Dec;161:60-70

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3 Spatial distribution of AQPs that function as H 2 O 2 channels. Immunostaining of AQPs was performed to investigate the contribution of AQPs to H 2 O 2 permeability. A: Immunostaining of AQP3 in HeLa and SAS rho 0 cells. B: Relative fluorescence intensity of AQP3 in HeLa and SAS rho 0 cells. C: Immunostaining of AQP5. D: Relative intensity of AQP5. E: Immunostaining of AQP8. F: Relative fluorescence intensity of AQP8. In HeLa and SAS rho 0 cells, AQPs were strongly expressed in the plasma membrane, and average expression intensities were significantly higher than in parental cells. **: p < 0.01 using Student's t- test (vs. parent). Fig. 3