Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-77708 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aquaporin 3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat kidney membranes with Aquaporin 3 polyclonal antibody (Product # PA5-77708) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Aquaporin 3 Polyclonal Antibody (Product # PA5-77708) and a 35kDa band corresponding to Aquaporin 3 was observed in all tissue lysates tested except Rat Skeletal Muscle [DOI: 10.1007/s00109-007-0272-4]. High molecular weight glycosylated forms (35-160 kDa) of Aquaporin 3 were also observed. Tissue extracts (30 µg lysate) of Rat Kidney (Lane 1), Rat Tongue (Lane 2) and Rat Skeletal Muscle (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:200 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). An uncharacterized band (*) was observed at ~22kDa in some tissue models.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Aquaporin 3 in paraffin-embedded rat colon. Samples were probed with Aquaporin 3 polyclonal antibody (Product # PA5-77708). Images show absorptive cells that are present in the luminal epithelium and in the superior third of the intestinal glands (arrows). Images also show the mucosa (M), submucosa (SM) andmuscularis externa(ME).