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- Product number
- PA5-78811 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aquaporin 3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references Unsaturated fatty acid-enriched extract from Hippophae rhamnoides seed reduces skin dryness through up-regulating aquaporins 3 and hyaluronan synthetases 2 expressions.
Yao Q, Jia T, Qiao W, Gu H, Kaku K
Journal of cosmetic dermatology 2021 Jan;20(1):321-329
Journal of cosmetic dermatology 2021 Jan;20(1):321-329
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Aquaporin 3 on HeLa cells. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with Aquaporin 3 polyclonal antibody (Product# PA5-78811) with a dilution of 1 µg/mL (overnight at 4°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 3 Figure SBT seed oil treatment evidently up-regulated AQP3 and HAS2 protein expressions based on result of IF. A, Immunohistofluorescence analysis was executed for AQP3 in NHEK cells. B, Relative optical densities of AQP3. C, IF analysis was executed to HAS2 in NHEK cells. D, Relative optical densities of HAS2. Magnification 20x, data are expressed as mean +- standard deviation (SD). Compared with the negative control, ** represents P < .01. HAS2, Hyaluronic acid synthase 2. Green represents AQP3 protein, red represents HAS2 protein, and blue represents DAPI stained cell nucleus
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 5 Figure Treating with SBT seed oil remarkably enhanced AQP3 and HAS2 protein expressions in reconstructed skin model compared with NC. A, IF analysis was used to investigate protein level of AQP3 and HAS2, in reconstructed skin model. Green represents AQP3 protein, red represents HAS2 protein, and blue represents DAPI stained cell nucleus. B, Relative optical densities of AQP3 and HAS2. Magnification 20x, data are expressed as mean +- standard deviation (SD). Compared with the negative control, * represents P < .01
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 4 Figure SBT seed oil treatment evidently up-regulated AQP3 and HAS2 protein expressions based on result of Western blot. A, Western blot analysis was used to investigate protein level of AQP3 in NHEK cells. B, Relative optical densities of AQP3. C, Western blot analysis was used to investigate protein level of HAS2 in NHEK cells. D, Relative optical densities of HAS2. Data are expressed as mean +- standard deviation (SD). Compared with the negative control, * represents P < .05, ** represents P < .01
