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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-30763 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPP2R2D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: IMR32, mouse brain, rat brain. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (95%), Xenopus laevis (95%), Chicken (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references CDC25B partners with PP2A to induce AMPK activation and tumor suppression in triple negative breast cancer.
Cairns J, Ly RC, Niu N, Kalari KR, Carlson EE, Wang L
NAR cancer 2020 Dec;2(4):zcaa039
NAR cancer 2020 Dec;2(4):zcaa039
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. CDC25B interacts directly with the PP2A-C subunit. ( A ) Top: GST-CDC25B was used to pull down purified His-PP2A-A, His-B56delta, His-PP2A-C, His-PP2A-A + B56delta, His-PP2A-A + PP2A-C, His-B56delta + PP2A-C or His-PP2A-A + B56delta + PP2A-C. Bottom: GST immunoblot of SDS-PAGE gel demonstrating the amount of CDC25B used for pull-down experiments. ( B and C ) The CDC25B-B56delta interaction in Neg or PP2A-C KD cells. BT549 and HS578T cells were transfected with Neg or siPP2A-C. Forty-eight hours later, cell lysates were subjected to IP with control IgG or anti-CDC25B antibody examining the CDC25B-B56delta interaction. Reciprocal IP with B56delta antibody was also performed to examine the CDC25B-B56delta interaction. The immunoprecipitates were blotted with the indicated antibodies. ( D ) B56delta, not B55delta, interacts with CDC25B. BT549 cells were transfected with siB56delta. Forty-eight hours later, cell lysates were subjected to IP with control IgG or anti-CDC25B antibody examining the CDC25B-B55delta interaction. Reciprocal IP with B55delta antibody was also performed to exam the CDC25B-B55delta interaction. The immunoprecipitates were blotted with the indicated antibodies. Western blot analysis demonstrates the protein level of B55delta in Neg or B56delta KD cells. Data information: all data presented are a representation of N = 3 independent experiments.
