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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-34915 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAD26L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG, C9orf102 transfected 293T lysates. Predicted reactivity: Bovine (80%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RAD26L Polyclonal Antibody (Product # PA5-34915). Sample (30 µg of whole cell lysate). Lane A: Non-transfected 293T lysates. Lane B: C9orf102 transfected 293T lysates. 10% SDS PAGE. RAD26L Polyclonal Antibody (Product # PA5-34915) diluted at 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RAD26L Polyclonal Antibody (Product # PA5-34915). Sample (30 µg of whole cell lysate). Lane A: U87-MG . 10% SDS PAGE. RAD26L Polyclonal Antibody (Product # PA5-34915) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C9orf102 antibody detects C9orf102 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. C9orf102 antibody (Product # PA5-34915) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
