TA501687

antibody from OriGene

Targeting: CBWD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501687

Provider

OriGene

Proper citation

OriGene Cat#TA501687, RRID:AB_11142692

Product name

Anti-CBWD1 mouse monoclonal antibody, clone OTI2D2 (formerly 2D2)

Antibody type

Monoclonal

Description

Anti-CBWD1 mouse monoclonal antibody, clone OTI2D2 (formerly 2D2)

Host

Mouse

Conjugate

Unconjugated

Epitope

CBWD1

Isotype

IgG

Antibody clone number

OTI2D2

Vial size

100 µl

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CBWD1 monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBWD1 (RC222790, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBWD1.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-CBWD1 mouse monoclonal antibody (TA501687) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CBWD1(RC222790).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC222790 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CBWD1 antibody(TA501687), and then analyzed by flow cytometry.

Validation comment

FC