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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA5-29522 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 31 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa. Predicted reactivity: Mouse (89%), Rat (87%), Pig (87%), Chimpanzee (100%), Bovine (86%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.85 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Cytokeratin 31 Polyclonal Antibody detects KRT31 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KRT31 protein stained by Cytokeratin 31 Polyclonal Antibody (Product # PA5-29522) diluted at 1:500. Blue: Hoechst 33342 staining.
