24766-1-AP

antibody from Proteintech Group

Targeting: INSIG2

Provider product page for 24766-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [22]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 24766-1-AP - Provider product page
Provider: Proteintech Group
Product name: INSIG2 antibody
Antibody type: Polyclonal
Description: KD/KO validated INSIG2 antibody (Cat. #24766-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

INSIG2 protein structure - 24766-1-AP shown in red.

Submitted references Iron overload reprogramming lipid metabolism through the IRP1-SCAP axis in fibroblast-like synoviocytes aggravates bone destruction in rheumatoid arthritis.

Liu Y, Fang L, Wang M, Zhong S, Xiong Y, Hou Y, Ren J, Li Y, Zhu W, Meng X, Lu C, Pan Y
Experimental & molecular medicine 2026 May 1;

KDM5B cooperates with CRL4B complex to promote the tumorigenesis of ER+ breast cancer via regulating cholesterol metabolism.

Yang Y, Gao T, Yuan B, Hao X, Huo M, Hu T, Ma T, Zhang M, Zhang D, Teng X, Yu H, Huang W, Zhang J, Wang Y
Cell death & disease 2026 Feb 7;17(1):207

Xia Ku Cao Paste restores intestinal microbiota homeostasis and improves hepatic metabolism disturbances to alleviate hyperlipidemia.

Du Z, Wang X, Xu L, Hu Z, Liu M, Min X, Hu J, Meng Y, Ye L, Liu S, Shi Z
Phytomedicine : international journal of phytotherapy and phytopharmacology 2026 Apr;153:158010

RORα fine-tunes the circadian control of hepatic triglyceride synthesis and gluconeogenesis.

Monnier C, Ganbold M, Auclair M, Roblot N, Boutin AB, Boutin PK, Fève B, Antoine B
Scientific reports 2025 Mar 26;15(1):10464

HSPA8 dampens SCAP/INSIG split and SREBP activation by reducing PKR-mediated INSIG phosphorylation.

Yang G, Ma C, Chen Y, Xiang J, Li L, Li Y, Kang L, Liang Z, Yang S
Cell reports 2025 Mar 25;44(3):115339

The SDF-1α/MTDH axis inhibits ferroptosis and promotes the formation of anti-VEGF-resistant choroidal neovascularization by facilitating the nuclear translocation of SREBP1.

Zou R, Zhang X, Dai X, Yuan Y, Dai J, Yuan F
Cell biology and toxicology 2025 Jul 17;41(1):118

A cholesterol-responsive hepatic tRNA-derived small RNA regulates cholesterol homeostasis and atherosclerosis development.

Li X, Hernandez R, Zhang X, Tang S, Yuan X, Wu J, Pham K, Rawal HC, Heinrich EC, Zhang S, Chen Q, Zhou T, Zhou C
Nature communications 2025 Dec 15;16(1):11043

Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma.

Zhu J, Xiong Y, Zhang Y, Liang H, Cheng K, Lu Y, Cai G, Wu Y, Fan Y, Chen X, Zhu H, Ding Z, Zhang W
Cancer communications (London, England) 2025 Aug;45(8):1038-1071

Effects of Cholesterol Metabolism on Corneal Endothelial Dysfunction in Patients With Type 1 Diabetes.

Ren Z, Li Z, Dou S, Zhao H, Xie L
Investigative ophthalmology & visual science 2025 Aug 1;66(11):9

Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis.

Yang X, Gu C, Cai J, Li F, He X, Luo L, Xiao W, Hu B, Hu J, Qian H, Ren S, Zhang L, Zhu X, Yang L, Yang J, Yang Z, Zheng Y, Huang X, Wang Z
Redox biology 2024 Feb;69:103002

Porcine reproductive and respiratory syndrome virus activates lipid synthesis through a ROS-dependent AKT/PCK1/INSIG/SREBPs axis.

Ma YX, Han YQ, Wang PZ, Wang MY, Yang GY, Li JL, Wang J, Chu BB
International journal of biological macromolecules 2024 Dec;282(Pt 1):136720

Insulin-induced gene 2 protects against hepatic ischemia-reperfusion injury via metabolic remodeling.

Wu Y, Li C, Khan AA, Chen K, Su R, Xu S, Sun Y, Gao F, Wang K, Wang X, Lian Z, Wang S, Yu M, Hu X, Yang F, Zheng S, Qiu N, Liu Z, Xu X
Journal of translational medicine 2023 Oct 19;21(1):739

A Multi-Level Systems Biology Analysis of Aldrin's Metabolic Effects on Prostate Cancer Cells.

Bedia C, Dalmau N, Nielsen LK, Tauler R, Marín de Mas I
Proteomes 2023 Mar 23;11(2)

In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion.

Vatandaslar H, Garzia A, Meyer C, Godbersen S, Brandt LTL, Griesbach E, Chao JA, Tuschl T, Stoffel M
Nature communications 2023 Jun 9;14(1):3386

Alleviation of Limosilactobacillus reuteri in polycystic ovary syndrome protects against circadian dysrhythmia-induced dyslipidemia via capric acid and GALR1 signaling.

Li S, Zhai J, Chu W, Geng X, Wang D, Jiao L, Lu G, Chan WY, Sun K, Sun Y, Chen ZJ, Du Y
NPJ biofilms and microbiomes 2023 Jul 8;9(1):47

Insulin-induced gene 2 expression is associated with cervical adenocarcinoma malignant behavior.

Qian Y, Li X, Qiu J, Zhang R
Molecular biology reports 2023 Feb;50(2):1553-1563

Dickkopf-1 promotes vascular smooth muscle cell foam cell formation and atherosclerosis development through CYP4A11/SREBP2/ABCA1.

Li X, Zheng T, Zhang Y, Zhao Y, Liu F, Dai S, Liu X, Zhang M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Aug;37(8):e23048

The GR-gp78 Pathway is involved in Hepatic Lipid Accumulation Induced by Overexpression of 11β-HSD1.

Hu M, Han T, Pan Q, Ni D, Gao F, Wang L, Ren H, Zhang X, Jiao H, Wang Y, Dai D, Man Y, Tang W, Sun Y, Li W, Li J, Li G
International journal of biological sciences 2022;18(8):3107-3121

AGR2-induced cholesterol synthesis drives lovastatin resistance that is overcome by combination therapy with allicin.

Sheng N, Wang YQ, Wang CF, Jia MQ, Niu HM, Lu QQ, Wang YN, Feng D, Zheng XX, Yuan HQ
Acta pharmacologica Sinica 2022 Nov;43(11):2905-2916

CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.

Zeng H, Qin H, Liao M, Zheng E, Luo X, Xiao A, Li Y, Chen L, Wei L, Zhao L, Ruan XZ, Yang P, Chen Y
Molecular metabolism 2022 Mar;57:101428

The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.

Xu D, Wang Z, Xia Y, Shao F, Xia W, Wei Y, Li X, Qian X, Lee JH, Du L, Zheng Y, Lv G, Leu JS, Wang H, Xing D, Liang T, Hung MC, Lu Z
Nature 2020 Apr;580(7804):530-535

p38α/S1P/SREBP2 activation by the SAM-competitive EZH2 inhibitor GSK343 limits its anticancer activity but creates a druggable vulnerability in hepatocellular carcinoma.

Yang PM, Hong YH, Hsu KC, Liu TP
American journal of cancer research 2019;9(10):2120-2139

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: rat liver tissue were subjected to SDS PAGE followed by western blot with 24766-1-AP( INSIG2 Antibody) at dilution of 1:1000
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The INSIG2 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human INSIG2. This antibody recognizes human, mouse, rat antigen. The INSIG2 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 24766-1-AP.pdf

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