Explore
Validate
Learn
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-25593 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HAS2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references GNA14 stimulation of KLF7 promotes malignant growth of endometrial cancer through upregulation of HAS2.
Increased Hyaluronan and TSG-6 in Association with Neuropathologic Changes of Alzheimer's Disease.
Wang J, Teng F, Chai H, Zhang C, Liang X, Yang Y
BMC cancer 2021 Apr 23;21(1):456
BMC cancer 2021 Apr 23;21(1):456
Increased Hyaluronan and TSG-6 in Association with Neuropathologic Changes of Alzheimer's Disease.
Reed MJ, Damodarasamy M, Pathan JL, Chan CK, Spiekerman C, Wight TN, Banks WA, Day AJ, Vernon RB, Keene CD
Journal of Alzheimer's disease : JAD 2019;67(1):91-102
Journal of Alzheimer's disease : JAD 2019;67(1):91-102
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of K562 cells using a HAS2 polyclonal antibody (Product # PA5-25593) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 KLF7 upregulation of HAS2 promotes UCEC cell proliferation and migration. a-d Immunoblotting analysis of HAS2 and cell viability were checked in Ctrl and HAS2 overexpressed KLE cells ( a ), in siCtrl, siKLF7 and siKLF7 + HAS2 KLE cells ( b ), in siCtrl and siHAS2 KLE cells ( c ), and in Ctrl, KLF7 and KLF7 + siHAS2 KLE cells ( d ). e CCK8 analysis of proliferation in Ctrl, KLF7 and KLF7 + siHAS2 KLE cells. f Colony formation was assessed in Ctrl, KLF7 and KLF7 + siHAS2 KLE cells. g Migration was assessed by wound healing in Ctrl, KLF7 and KLF7 + siHAS2 KLE cells. * p < 0.05. ** p < 0.01. *** p < 0.001. Results are mean +- SEM ( n = 3)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7 KLF7 promotes tumorigenesis of UCEC cells through upregulation of HAS2. Equal number of shCtrl and shKLF7 Hec-1-B cells were subcutaneously implanted into female nude mice. Tumors were photographed after sacrifice. a Macroscopic images of tumors derived from shCtrl and shKLF7 Hec-1-B cells in nude mice. b Tumor weight was measured. c Immunoblotting analysis of KLF7 and HAS2 in shCtrl and shKLF7 tumor tissues. Equal number of Ctrl, KLF7, KLF7 + shHAS2 Hec-1-B cells were subcutaneously implanted into female nude mice. Tumors were photographed after sacrifice. d Macroscopic images of tumors derived from Ctrl, KLF7, KLF7 + shHAS2 Hec-1-B cells in nude mice. e Tumor weight was measured. * p < 0.05. ** p < 0.01
