HPA003156

antibody from Atlas Antibodies

Targeting: ATP6AP2 APT6M8-9, ATP6IP2, ATP6M8-9, M8-9, PRR, RENR

Western blot (Enhanced validation)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA003156

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA003156, RRID:AB_1078245

Product name

Anti-ATP6AP2

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human ATP6AP2, Gene description: ATPase, H+ transporting, lysosomal accessory protein 2, Alternative Gene Names: APT6M8-9, ATP6IP2, ATP6M8-9, M8-9, PRR, RENR, Validated applications: IHC, WB, Uniprot ID: O75787, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Renal Expression and Localization of the Receptor for (Pro)renin and Its Ligands in Rodent Models of Diabetes, Metabolic Syndrome, and Age-Dependent Focal and Segmental Glomerulosclerosis

Iacobini C, Vitale M, Sentinelli F, Haxhi J, Pugliese G, Menini S
International Journal of Molecular Sciences 2024;25(4):2217

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OXGR1-Dependent (Pro)Renin Receptor Upregulation in Collecting Ducts of the Clipped Kidney Contributes to Na+ Balance in Goldblatt Hypertensive Mice

Cárdenas P, Nuñez-Allimant C, Silva K, Cid-Salinas C, León A, Vallotton Z, Lorca R, Oliveira L, Casarini D, Céspedes C, Prieto M, Gonzalez A
International Journal of Molecular Sciences 2024;25(18):10045

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(Pro)renin receptor mediates tubular epithelial cell pyroptosis in diabetic kidney disease via DPP4-JNK pathway

Xie S, Song S, Liu S, Li Q, Zou W, Ke J, Wang C
Journal of Translational Medicine 2024;22(1)

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Inhibition of Wnt/β-catenin signaling reduces renal fibrosis in murine glycogen storage disease type Ia

Lee C, Pratap K, Zhang L, Chen H, Gautam S, Arnaoutova I, Raghavankutty M, Starost M, Kahn M, Mansfield B, Chou J
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2024;1870(1):166874

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ATP6AP2, a regulator of LRP6/β-catenin protein trafficking, promotes Wnt/β-catenin signaling and bone formation in a cell type dependent manner

Xiong L, Guo H, Pan J, Ren X, Lee D, Chen L, Mei L, Xiong W
Bone Research 2024;12(1)

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(Pro)Renin Receptor Decoy Peptide PRO20 Protects against Oxidative Renal Damage Induced by Advanced Oxidation Protein Products

Fang H, Yang T, Zhou B, Li X
Molecules 2023;28(7):3017

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(Pro)renin receptor promotes colorectal cancer progression through inhibiting the NEDD4L-mediated Wnt3 ubiquitination and modulating gut microbiota

Wang J, Ding Y, Li D, Zhu N, Nishiyama A, Yuan Y
Cell Communication and Signaling 2023;21(1)

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Endothelial cell polarity and extracellular matrix composition require functional ATP6AP2 during developmental and pathological angiogenesis

Patel N, K C R, Blanks A, Li Y, Prieto M, Meadows S
JCI Insight 2022;7(19)

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Intramembrane client recognition potentiates the chaperone functions of calnexin

Bloemeke N, Meighen‐Berger K, Hitzenberger M, Bach N, Parr M, Coelho J, Frishman D, Zacharias M, Sieber S, Feige M
The EMBO Journal 2022;41(24)

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(Pro)renin Receptor Regulates Phosphate Homeostasis in Rats via Releasing Fibroblast Growth Factor-23

Lu A, Pu M, Mo S, Su J, Hu J, Li C, Wang W, Yang T
Frontiers in Physiology 2022;13

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(Pro)renin receptor antagonist PRO20 attenuates nephrectomy‐induced nephropathy in rats via inhibition of intrarenal RAS and Wnt/β‐catenin signaling

Wang Y, Wang Y, Xue K, Wang H, Zhou J, Gao F, Li C, Yang T, Fang H
Physiological Reports 2021;9(11)

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Mutagenesis of the Cleavage Site of Pro Renin Receptor Abrogates Angiotensin II-Induced Hypertension in Mice

Wang F, Chen Y, Zou C, Luo R, Yang T
Hypertension 2021;78(1):115-127

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Site-1 Protease-Derived Soluble (Pro)Renin Receptor Contributes to Angiotensin II–Induced Hypertension in Mice

Feng Y, Peng K, Luo R, Wang F, Yang T
Hypertension 2021;77(2):405-416

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The (pro)renin receptor (ATP6ap2) facilitates receptor-mediated endocytosis and lysosomal function in the renal proximal tubule

Figueiredo M, Daryadel A, Sihn G, Müller D, Popova E, Rouselle A, Nguyen G, Bader M, Wagner C
Pflügers Archiv - European Journal of Physiology 2021;473(8):1229-1246

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The prorenin receptor and its soluble form contribute to lipid homeostasis

Gatineau E, Arthur G, Poupeau A, Nichols K, Spear B, Shelman N, Graf G, Temel R, Yiannikouris F
American Journal of Physiology-Endocrinology and Metabolism 2021;320(3):E609-E618

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Knockout of Nephron ATP6AP2 Impairs Proximal Tubule Function and Prevents High-Fat Diet-Induced Obesity in Male Mice

Culver S, Akhtar S, Rountree-Jablin C, Keller S, Cathro H, Gildea J, Siragy H
Endocrinology 2021;162(12)

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(Pro)renin Receptor Is a Novel Independent Prognostic Marker in Invasive Urothelial Carcinoma of the Bladder

Larrinaga G, Calvete-Candenas J, Solano-Iturri J, Martín A, Pueyo A, Nunes-Xavier C, Pulido R, Dorado J, López J, Angulo J
Cancers 2021;13(22):5642

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Diuretic Action of Apelin-13 Mediated by Inhibiting cAMP/PKA/sPRR Pathway

Chen Y, Xu C, Hu J, Deng M, Qiu Q, Mo S, Du Y, Yang T
Frontiers in Physiology 2021;12

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Novel regulation of renal gluconeogenesis by Atp6ap2 in response to high fat diet via PGC1-α/AKT-1 pathway

Akhtar S, Culver S, Siragy H
Scientific Reports 2021;11(1)

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High glucose induces trafficking of prorenin receptor and stimulates profibrotic factors in the collecting duct

Gogulamudi V, Arita D, Bourgeois C, Jorgensen J, He J, Wimley W, Satou R, Gonzalez A, Prieto M
Scientific Reports 2021;11(1)

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Cancer Stem Cells in Metastatic Head and Neck Cutaneous Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System

Siljee S, Buchanan O, Brasch H, Bockett N, Patel J, Paterson E, Purdie G, Davis P, Itinteang T, Tan S
Cells 2021;10(2):243

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(Pro)renin receptor decoy peptide PRO20 protects against adriamycin-induced nephropathy by targeting the intrarenal renin-angiotensin system

Luo R, Yang K, Wang F, Xu C, Yang T
American Journal of Physiology-Renal Physiology 2020;319(5):F930-F940

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ELABELA antagonizes intrarenal renin-angiotensin system to lower blood pressure and protects against renal injury

Xu C, Wang F, Chen Y, Xie S, Sng D, Reversade B, Yang T
American Journal of Physiology-Renal Physiology 2020;318(5):F1122-F1135

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Lipid droplet velocity is a microenvironmental sensor of aggressive tumors regulated by V-ATPase and PEDF

Nardi F, Fitchev P, Brooks K, Franco O, Cheng K, Hayward S, Welte M, Crawford S
Laboratory Investigation 2019;99(12):1822-1834

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(Pro)renin receptor contributes to renal mitochondria dysfunction, apoptosis and fibrosis in diabetic mice

Li C, Matavelli L, Akhtar S, Siragy H
Scientific Reports 2019;9(1)

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Expression of Components of the Renin-Angiotensin System in Pyogenic Granuloma

Papali'i-Curtin J, Brasch H, van Schaijik B, de Jongh J, Marsh R, Tan S, Itinteang T
Frontiers in Surgery 2019;6

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(Pro)renin receptor regulates lung development via the Wnt/β-catenin signaling pathway

Liu J, Zhou Y, Liu Y, Li L, Chen Y, Liu Y, Feng Y, Yosypiv I, Song R, Peng H
American Journal of Physiology-Lung Cellular and Molecular Physiology 2019;317(2):L202-L211

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(Pro)renin receptor contributes to pregnancy-induced sodium-water retention in rats via activation of intrarenal RAAS and α-ENaC

Fu Z, Hu J, Zhou L, Chen Y, Deng M, Liu X, Su J, Lu A, Fu X, Yang T
American Journal of Physiology-Renal Physiology 2019;316(3):F530-F538

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Pro-renin receptor suppresses mitochondrial biogenesis and function via AMPK/SIRT-1/ PGC-1α pathway in diabetic kidney

Sen U, Akhtar S, Siragy H
PLOS ONE 2019;14(12):e0225728

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Radiation Nephropathy in a Nonhuman Primate Model of Partial-Body Irradiation With Minimal Bone Marrow Sparing—Part 2: Histopathology, Mediators, and Mechanisms

Parker G, Cohen E, Li N, Takayama K, Farese A, MacVittie T
Health Physics 2019;116(3):409-425

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(Pro)renin Receptor Expression Increases throughout the Colorectal Adenoma—Adenocarcinoma Sequence and It Is Associated with Worse Colorectal Cancer Prognosis

Beitia M, Solano-Iturri J, Errarte P, Calvete-Candenas J, Loizate A, Etxezarraga M, Sanz B, Larrinaga G
Cancers 2019;11(6):881

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(Pro)renin Receptor-Dependent Induction of Profibrotic Factors Is Mediated by COX-2/EP4/NOX-4/Smad Pathway in Collecting Duct Cells

Reyes-Martinez C, Nguyen Q, Kassan M, Gonzalez A
Frontiers in Pharmacology 2019;10

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Downregulated microRNA‑133a induces HUVECs injury: Potential role of the (pro) renin receptor in angiotensin�II‑dependent hypertension

Liu B, Lan M, Wei H, Zhang D, Liu J, Teng J
Molecular Medicine Reports 2019

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Loss-of-function mutations in ATP6AP1 and ATP6AP2 in granular cell tumors

Pareja F, Brandes A, Basili T, Selenica P, Geyer F, Fan D, Da Cruz Paula A, Kumar R, Brown D, Gularte-Mérida R, Alemar B, Bi R, Lim R, de Bruijn I, Fujisawa S, Gardner R, Feng E, Li A, da Silva E, Lozada J, Blecua P, Cohen-Gould L, Jungbluth A, Rakha E, Ellis I, Edelweiss M, Palazzo J, Norton L, Hollmann T, Edelweiss M, Rubin B, Weigelt B, Reis-Filho J
Nature Communications 2018;9(1)

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Essential role of ATP6AP2 enrichment in caveolae/lipid raft microdomains for the induction of neuronal differentiation of stem cells

Makdissy N, Haddad K, AlBacha J, Chaker D, Ismail B, Azar A, Oreibi G, Ayoub D, Achkar I, Quilliot D, Fajloun Z
Stem Cell Research & Therapy 2018;9(1)

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Role of (pro)renin receptor in albumin overload-induced nephropathy in rats

Fang H, Deng M, Zhang L, Lu A, Su J, Xu C, Zhou L, Wang L, Ou J, Wang W, Yang T
American Journal of Physiology-Renal Physiology 2018;315(6):F1759-F1768

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Elevated (Pro)renin Receptor Expression Contributes to Maintaining Aerobic Metabolism in Growth Hormone Deficiency

Seki Y, Yatabe M, Suda C, Morimoto S, Ichihara A
Journal of the Endocrine Society 2018;2(3):252-265

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Atp6ap2 ablation in adult mice impairs viability through multiple organ deficiencies

Wendling O, Champy M, Jaubert S, Pavlovic G, Dubos A, Lindner L, Jacobs H, Mark M, Combe R, Da Cruz I, Luche H, Mudgett J, Rosahl T, Sorg T, Malissen M, Reilly P, Hérault Y
Scientific Reports 2017;7(1)

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Embryonic Stem Cell-Like Population in Dupuytren’s Disease Expresses Components of the Renin-Angiotensin System

On N, Koh S, Brasch H, Dunne J, Armstrong J, Tan S, Itinteang T
Plastic and Reconstructive Surgery - Global Open 2017;5(7):e1422

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(Pro)renin receptor activation increases profibrotic markers and fibroblast‐like phenotype through MAPK‐dependent ROS formation in mouse renal collecting duct cells

Gonzalez A, Zamora L, Reyes‐Martinez C, Salinas‐Parra N, Roldan N, Cuevas C, Figueroa S, Gonzalez‐Vergara A, Prieto M
Clinical and Experimental Pharmacology and Physiology 2017;44(11):1134-1144

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Cancer Stem Cells in Moderately Differentiated Lip Squamous Cell Carcinoma Express Components of the Renin–Angiotensin System

Ram R, Brasch H, Dunne J, Davis P, Tan S, Itinteang T
Frontiers in Surgery 2017;4

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NF-κB-dependent upregulation of (pro)renin receptor mediates high-NaCl-induced apoptosis in mouse inner medullary collecting duct cells

Su J, Liu X, Xu C, Lu X, Wang F, Fang H, Lu A, Qiu Q, Li C, Yang T
American Journal of Physiology-Cell Physiology 2017;313(6):C612-C620

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Osteoblastic Lrp4 promotes osteoclastogenesis by regulating ATP release and adenosine-A2AR signaling

Xiong L, Jung J, Guo H, Pan J, Sun X, Mei L, Xiong W
Journal of Cell Biology 2017;216(3):761-778

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Mutations in the X-linked ATP6AP2 cause a glycosylation disorder with autophagic defects

Rujano M, Cannata Serio M, Panasyuk G, Péanne R, Reunert J, Rymen D, Hauser V, Park J, Freisinger P, Souche E, Guida M, Maier E, Wada Y, Jäger S, Krogan N, Kretz O, Nobre S, Garcia P, Quelhas D, Bird T, Raskind W, Schwake M, Duvet S, Foulquier F, Matthijs G, Marquardt T, Simons M
Journal of Experimental Medicine 2017;214(12):3707-3729

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Cancer stem cells in moderately differentiated oral tongue squamous cell carcinoma express components of the renin–angiotensin system

Itinteang T, Dunne J, Chibnall A, Brasch H, Davis P, Tan S
Journal of Clinical Pathology 2016;69(10):942-945

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Human placental renin–angiotensin system in normotensive and pre‐eclamptic pregnancies at high altitude and after acute hypoxia–reoxygenation insult

Kurlak L, Mistry H, Cindrova‐Davies T, Burton G, Pipkin F
The Journal of Physiology 2016;594(5):1327-1340

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Expression of the Components of the Renin–Angiotensin System in Venous Malformation

Siljee S, Keane E, Marsh R, Brasch H, Tan S, Itinteang T
Frontiers in Surgery 2016;3

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Cancer Stem Cells in Moderately Differentiated Buccal Mucosal Squamous Cell Carcinoma Express Components of the Renin–Angiotensin System

Featherston T, Yu H, Dunne J, Chibnall A, Brasch H, Davis P, Tan S, Itinteang T
Frontiers in Surgery 2016;3

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Vacuolar ATPase in Phagosome-Lysosome Fusion

Kissing S, Hermsen C, Repnik U, Nesset C, von Bargen K, Griffiths G, Ichihara A, Lee B, Schwake M, De Brabander J, Haas A, Saftig P
Journal of Biological Chemistry 2015;290(22):14166-14180

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Increased expression of (pro)renin receptor does not cause hypertension or cardiac and renal fibrosis in mice

Rosendahl A, Niemann G, Lange S, Ahadzadeh E, Krebs C, Contrepas A, van Goor H, Wiech T, Bader M, Schwake M, Peters J, Stahl R, Nguyen G, Wenzel U
Laboratory Investigation 2014;94(8):863-872

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Angiotensin II Increases the Expression of (Pro)Renin Receptor During Low-Salt Conditions

Gonzalez A, Womack J, Liu L, Prieto M, Seth D
The American Journal of the Medical Sciences 2014;348(5):416-422

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The (pro)renin receptor mediates constitutive PLZF-independent pro-proliferative effects which are inhibited by bafilomycin but not genistein

KIRSCH S, SCHREZENMEIER E, KLARE S, ZAADE D, SEIDEL K, SCHMITZ J, BERNHARD S, LAUER D, SLACK M, GOLDIN-LANG P, UNGER T, ZOLLMANN F, FUNKE-KAISER H
International Journal of Molecular Medicine 2014;33(4):795-808

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Renal medullary cyclooxygenase-2 and (pro)renin receptor expression during angiotensin II-dependent hypertension

Gonzalez A, Green T, Luffman C, Bourgeois C, Gabriel Navar L, Prieto M
American Journal of Physiology-Renal Physiology 2014;307(8):F962-F970

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Distinct Signal Transduction Pathways Downstream of the (P)RR Revealed by Microarray and ChIP-chip Analyses

Datta P, Zaade D, Schmitz J, Benke E, Klare S, Seidel K, Kirsch S, Goldin-Lang P, Zollmann F, Unger T, Funke-Kaiser H
PLoS ONE 2013;8(3):e57674

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Soluble Form of the (Pro)Renin Receptor Is Augmented in the Collecting Duct and Urine of Chronic Angiotensin II–Dependent Hypertensive Rats

Gonzalez A, Lara L, Luffman C, Seth D, Prieto M
Hypertension 2011;57(4):859-864

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Enhancement of renin and prorenin receptor in collecting duct of Cyp1a1-Ren2 rats may contribute to development and progression of malignant hypertension

Prieto M, Williams D, Liu L, Kavanagh K, Mullins J, Mitchell K
American Journal of Physiology-Renal Physiology 2011;300(2):F581-F588

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATP6AP2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Sample type

Human

Protocol

Protocol