Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ANR-162-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NGL-2/LRRC4 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysates: - 1. Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (1:200). 2. Anti-NGL-2/LRRC4 (extracellular) Antibody , preincubated with NGL-2/LRRC4 (extracellular) Blocking Peptide (BLP-NR162).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain synaptosome preparation (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1-2. Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (1:200). 3-4. Anti-NGL-2/LRRC4 (extracellular) Antibody , preincubated with NGL-2/LRRC4 (extracellular) Blocking Peptide (BLP-NR162).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of NGL-2/LRRC4 in mouse cortex. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. NGL-2/LRRC4 immunoreactivity (green) appears in cortical neurons (arrows). B. Pre-incubation of the Antibody with NGL-2/LRRC4 (extracellular) Blocking Peptide (BLP-NR162), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of NGL-2/LRRC4 in rat hypothalamus. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NGL-2/LRRC4 (extracellular) Antibody (#ANR-162), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. NGL-2/LRRC4 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the Antibody with NGL-2/LRRC4 (extracellular) Blocking Peptide (BLP-NR162), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = third cerebral ventricle.
