16409-1-AP

antibody from Proteintech Group

Targeting: BGN DSPG1, SLRR1A

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16409-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16409-1-AP, RRID:AB_10804656

Product name

Biglycan antibody

Antibody type

Polyclonal

Description

Biglycan antibody (Cat. #16409-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat, pig and has been validated for the following applications: IF, IHC, WB, ELISA.

Reactivity

Human, Mouse, Rat, Porcine

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Enhancer-mediated NR2F2 recruitment activates BGN to promote tumor growth and shape tumor microenvironment in papillary thyroid cancer.

Tao M, Ruan X, Yu J, Dong Q, Luo W, Zhang W, Tian M, Hou X, Hu L, Zhao J, Li D, Hao J, Wei S, Zheng X, Gao M
Theranostics 2026;16(1):298-324

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BGN/MDK Axis in the Melanoma Tumor Microenvironment Strengthens Tumor Malignancy by Modulating Cancer Cells and Cancer-Associated Fibroblasts Crosstalk.

Shi HZ, Wu MY, Liu JQ, Tian C, Ma L, Zhou XM, Sun ZH, Xu ZY, Zhang RD, Ye SY, Huang LM, Wang Y, Xiong JS, Bu WB, Cheng XF, Sun JF, Chen H
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 May;13(28):e14590

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Efficacy and Potential Mechanisms of Yi-Ping Lin Circum-Knee Acupuncture in the Treatment of Knee Osteoarthritis.

Huang L, Deng X, Jin Y, Li J, Wu X, Xiong Y, Guo S, Yan S, Lu S, Chen D
Journal of pain research 2025;18:5891-5906

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Association of High Tumor-Stroma Ratio with Prostate Cancer Progression: Insights from Clinical and Genomic Data.

Xu W, Niu Q, Zhao K, Zhao H, Zhang L, Li W, Yan H, Dong Z
International journal of general medicine 2025;18:2599-2618

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Biglycan deficiency alleviates intestinal fibrosis through BMP-7-mediated Smad1/5/8 signaling.

Yu M, Si C, Xinjue H, Pan Y, Dai Y, Jin C, Han T, Yu C, Zhang J
Journal of Crohn's & colitis 2025 May 8;19(5)

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Vdr mediates Wnt signaling pathway to regulate odontoblasts differentiation during dentin apposition.

Wu Y, Zhu W, Wang L, Zhang W, Zhang K, Sun M, Guan J, Liu S, Liu Y
European journal of pharmacology 2025 Mar 15;991:177333

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Compressive Force Inhibits Osteogenic Differentiation of Dental Follicle Stem Cells Through Biglycan/Bone Morphogenetic Protein2/Smad1 Signaling Pathway.

Zhang R, Zhang J, Liu L, Wei T, Qin Y, Jiang C
Stem cells and development 2025 Jun;34(11-12):240-248

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Biglycan stimulates retinal pathological angiogenesis via up-regulation of CXCL12 expression in pericytes.

Liu M, Zhao P, Feng H, Yang Y, Zhang X, Chen E, Xiao H, Luo J, Chen H, Yin J, Lin M, Mao R, Zhu X, Li J, Fei P
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Jan 15;39(1):e70262

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BGN Secreted by Cancer-Associated Fibroblasts Promotes Esophageal Squamous Cell Carcinoma Progression via Activation of TLR4-Mediated Erk and NF-κB Signaling Pathways.

Yokoo H, Koma YI, Nomura N, Torigoe R, Omori M, Nakanishi T, Miyako S, Nakanishi T, Kodama T, Shigeoka M, Kakeji Y, Horie M
International journal of molecular sciences 2025 Dec 13;26(24)

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Integration of cell-type resolved spatial proteomics and transcriptomics reveals novel mechanisms in early ovarian cancer.

Metousis A, Kenny HA, Shimizu A, Schweizer L, Ben-Moshe S, Bilecz A, Krishnan R, Zhang J, Alcazar I, Kelliher L, Ravi M, Samantaray T, Richter S, Li Y, Wang J, Steigerwald S, Theis FJ, Rosenberger FA, Nordmann TM, Yamada SD, Lastra R, Mann M, Lengyel E
medRxiv : the preprint server for health sciences 2025 Aug 28;

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Single-cell sequencing unveils the transcriptomic landscape of castration-resistant prostate cancer-associated fibroblasts and their association with prognosis and immunotherapy response.

Qiu Y, Wang Y, Liu J, Sun K, Liu B, Hou Q
BMC cancer 2025 Apr 30;25(1):813

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FAM20B-Catalyzed Glycosylation Regulates the Chondrogenic and Osteogenic Differentiation of the Embryonic Condyle by Controlling IHH Diffusion and Release.

Chen X, Liu H, Huang Y, Li L, Jiang X, Liu B, Li N, Zhu L, Liu C, Xiao J
International journal of molecular sciences 2025 Apr 24;26(9)

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Proteomic analysis of extracellular vesicles derived from canine mammary tumour cell lines identifies protein signatures specific for disease state.

Gutierrez-Riquelme T, Karkossa I, Schubert K, Liebscher G, Packeiser EM, Nolte I, von Bergen M, Murua Escobar H, Aguilera-Rojas M, Einspanier R, Stein T
BMC veterinary research 2024 Oct 26;20(1):488

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Role of transglutaminase 2 in promoting biglycan synthesis in idiopathic gingival fibromatosis.

Ninomiya Y, Matsuda S, Suzuki S, Hirata-Tsuchiya S, Ueda T, Nakashima F, Yasuda K, Shimada S, Memida T, Yoshimoto T, Yamada S, Ouhara K, Mizuno N
BMC oral health 2024 Nov 21;24(1):1422

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Identification of ROS and KEAP1-related genes and verified targets of α-hederin induce cell death for CRC.

Wang G, Zhu ZM, Wang K
Drug development research 2024 May;85(3):e22200

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The role of vitamin D receptor in predentin mineralization and dental repair after injury.

Liu Y, Wu Y, Hu X, Sun Y, Zeng G, Wang Q, Liu S, Sun M
Cell and tissue research 2024 Jun;396(3):343-351

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Dermal papilla cell-secreted biglycan regulates hair follicle phase transit and regeneration by activating Wnt/β-catenin.

Zhu N, Yan J, Gu W, Yang Q, Lin E, Lu S, Cai B, Xia B, Liu X, Lin C
Experimental dermatology 2024 Jan;33(1):e14969

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Proteomic analysis of decellularized mice liver and kidney extracellular matrices.

Diedrich AM, Daneshgar A, Tang P, Klein O, Mohr A, Onwuegbuchulam OA, von Rueden S, Menck K, Bleckmann A, Juratli MA, Becker F, Sauer IM, Hillebrandt KH, Pascher A, Struecker B
Journal of biological engineering 2024 Feb 22;18(1):17

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Youthful small extracellular vesicles restore the function and reparative capacity of inflammatory-impaired periodontal ligament stem cells via delivering protein biglycan for bone regeneration.

Yang J, Su J, Sun Z, Song Y, Zhang Y, Zhang Z, Wei J, Shi X, Jiang N, Ge X
Journal of nanobiotechnology 2024 Aug 24;22(1):508

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Biglycan and reduced glycolysis are associated with breast cancer cell dormancy in the brain.

Sunderland A, Williams J, Andreou T, Rippaus N, Fife C, James F, Kartika YD, Speirs V, Carr I, Droop A, Lorger M
Frontiers in oncology 2023;13:1191980

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Biglycan as a mediator of proinflammatory response and target for MDS and sAML therapy.

Vaxevanis CK, Bauer M, Subbarayan K, Friedrich M, Massa C, Biehl K, Al-Ali HK, Wickenhauser C, Seliger B
Oncoimmunology 2023;12(1):2152998

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Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.

Lin Y, Chen M, Wang D, Yu Y, Chen R, Zhang M, Yu H, Huang X, Rao M, Wang Y, Li Y, Yan J, Yin P
Journal of proteome research 2023 Jun 2;22(6):1712-1722

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Establishment of a risk model by integrating hypoxia genes in predicting prognosis of esophageal squamous cell carcinoma.

Xiao W, Tang P, Sui Z, Han Y, Zhao G, Wu X, Yang Y, Zhu N, Gong L, Yu Z, Zhang H
Cancer medicine 2023 Jan;12(2):2117-2133

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Stroma biglycan expression can be a prognostic factor in prostate cancers.

Furumido J, Maishi N, Yanagawa-Matsuda A, Kikuchi H, Matsumoto R, Osawa T, Abe T, Matsuno Y, Shinohara N, Hida Y, Hida K
International journal of urology : official journal of the Japanese Urological Association 2023 Feb;30(2):147-154

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Dissecting the role of cancer-associated fibroblast-derived biglycan as a potential therapeutic target in immunotherapy resistance: A tumor bulk and single-cell transcriptomic study.

Zheng S, Liang JY, Tang Y, Xie J, Zou Y, Yang A, Shao N, Kuang X, Ji F, Liu X, Tian W, Xiao W, Lin Y
Clinical and translational medicine 2023 Feb;13(2):e1189

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Identification of hub genes and pathways in lung metastatic colorectal cancer.

Dai W, Guo C, Wang Y, Li Y, Xie R, Wu J, Yao B, Xie D, He L, Li Y, Huang H, Wang Y, Liu S
BMC cancer 2023 Apr 6;23(1):323

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Rejuvenation of tendon stem/progenitor cells for functional tendon regeneration through platelet-derived exosomes loaded with recombinant Yap1.

Lu J, Yang X, He C, Chen Y, Li C, Li S, Chen Y, Wu Y, Xiang Z, Kang J, Jiang G, Wang C, Diarra MD, He R, Feng G, Yan R
Acta biomaterialia 2023 Apr 15;161:80-99

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Identification of key genes and pathways revealing the central regulatory mechanism of brain-derived glucagon-like peptide-1 on obesity using bioinformatics analysis.

Shao Y, Tian J, Yang Y, Hu Y, Zhu Y, Shu Q
Frontiers in neuroscience 2022;16:931161

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Biglycan as a potential regulator of tumorgenicity and immunogenicity in K-RAS-transformed cells.

Subbarayan K, Massa C, Leisz S, Steven A, Bethmann D, Biehl K, Wickenhauser C, Seliger B
Oncoimmunology 2022;11(1):2069214

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Landscape of cancer-associated fibroblasts identifies the secreted biglycan as a protumor and immunosuppressive factor in triple-negative breast cancer.

Zheng S, Zou Y, Tang Y, Yang A, Liang JY, Wu L, Tian W, Xiao W, Xie X, Yang L, Xie J, Wei W, Xie X
Oncoimmunology 2022;11(1):2020984

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Expression and localization of CD63 in the intracellular vesicles of odontoblasts.

Matsuki-Fukushima M, Fujikawa K, Inoue S, Nakamura M
Histochemistry and cell biology 2022 Jun;157(6):611-622

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Biglycan Promotes Cancer Stem Cell Properties, NFκB Signaling and Metastatic Potential in Breast Cancer Cells.

Manupati K, Paul R, Hao M, Haas M, Bian ZC, Holm TM, Guan JL, Yeo SK
Cancers 2022 Jan 17;14(2)

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Effects of aging on the histology and biochemistry of rat tendon healing.

Lai F, Tang H, Wang J, Lu K, Bian X, Wang Y, Shi Y, Guo Y, He G, Zhou M, Zhang X, Zhou B, Zhang J, Chen W, Tang K
BMC musculoskeletal disorders 2021 Nov 15;22(1):949

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Biglycan, tumor endothelial cell secreting proteoglycan, as possible biomarker for lung cancer.

Morimoto H, Hida Y, Maishi N, Nishihara H, Hatanaka Y, Li C, Matsuno Y, Nakamura T, Hirano S, Hida K
Thoracic cancer 2021 May;12(9):1347-1357

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Quantitative Proteome of Infant Stenotic Ureters Reveals Extracellular Matrix Organization and Oxidative Stress Dysregulation Underlying Ureteropelvic Junction Obstruction.

Li Y, Chen Z, Zhang J, Zhang Q, He L, Xu M, Xu G, Geng H, Fang X
Proteomics. Clinical applications 2020 Nov;14(6):e2000030

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Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT-3 signalling pathway.

Wang Y, He G, Tang H, Shi Y, Kang X, Lyu J, Zhu M, Zhou M, Yang M, Mu M, Chen W, Zhou B, Zhang J, Tang K
Cell proliferation 2019 Jul;52(4):e12650

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Mmu-miR-185 depletion promotes osteogenic differentiation and suppresses bone loss in osteoporosis through the Bgn-mediated BMP/Smad pathway.

Cui Q, Xing J, Yu M, Wang Y, Xu J, Gu Y, Nan X, Ma W, Liu H, Zhao H
Cell death & disease 2019 Feb 20;10(3):172

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Expression and localization of the small proteoglycans decorin and biglycan in articular cartilage of Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency.

Wang M, Xue S, Fang Q, Zhang M, He Y, Zhang Y, Lammi MJ, Cao J, Chen J
Glycoconjugate journal 2019 Dec;36(6):451-459

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Biglycan-mediated upregulation of MHC class I expression in HER-2/neu-transformed cells.

Subbarayan K, Leisz S, Wickenhauser C, Bethmann D, Massa C, Steven A, Seliger B
Oncoimmunology 2018;7(4):e1373233

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 16409-1-AP(BGN antibody) at dilution of 1:800

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human renal cell carcinoma using 16409-1-AP(BGN antibody) at dilution of 1:50 (under 10x lens)

Sample type

cell line

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human renal cell carcinoma using 16409-1-AP(BGN antibody) at dilution of 1:50 (under 40x lens)

Sample type

cell line

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The Biglycan antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human Biglycan. This antibody recognizes human,mouse,rat antigen. The Biglycan antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

16409-1-AP.pdf

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