Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00000633-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000633-M01, RRID:AB_537141
- Product name
- Mouse Monoclonal Biglycan Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. BGN - biglycan
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The small leucine-rich proteoglycan BGN accumulates in CADASIL and binds to NOTCH3.
Zhang X, Lee SJ, Young MF, Wang MM
Translational stroke research 2015 Apr;6(2):148-55
Translational stroke research 2015 Apr;6(2):148-55
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Biglycan Antibody (4E1-1G7) [H00000633-M01] - Analysis of BGN expression in transfected 293T cell line by BGN monoclonal antibody (M01), clone 4E1-1G7.Lane 1: BGN transfected lysate(41.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Biglycan Antibody (4E1-1G7) [H00000633-M01] - BGN monoclonal antibody (M01), clone 4E1-1G7 Analysis of BGN expression in HepG2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Biglycan Antibody (4E1-1G7) [H00000633-M01] - Detection limit for recombinant GST tagged BGN is 3 ng/ml as a capture antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Biglycan Antibody (4E1-1G7) [H00000633-M01] - Detection limit for recombinant GST tagged BGN is 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Biglycan Antibody (4E1-1G7) [H00000633-M01] - Analysis of BGN transfected lysate using anti-BGN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BGN MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Biglycan Antibody (4E1-1G7) [H00000633-M01] - Analysis of monoclonal antibody to BGN on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. Antibody concentration 1 ~ 10 ug/ml.