NBP1-84971

antibody from Novus Biologicals

Targeting: BGN DSPG1, SLRR1A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-84971

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-84971, RRID:AB_11026502

Product name

Rabbit Polyclonal Biglycan Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human Biglycan antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Differential regulation of extracellular matrix protein expression in carcinoma-associated fibroblasts by TGF-β1 regulates cancer cell spreading but not adhesion.

Van Bockstal M, Lambein K, Van Gele M, De Vlieghere E, Limame R, Braems G, Van den Broecke R, Cocquyt V, Denys H, Bracke M, Libbrecht L, De Wever O
Oncoscience 2014;1(10):634-48

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Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.

Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Biglycan Antibody [NBP1-84971] - Analysis in human cell line U-138 MG.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Biglycan Antibody [NBP1-84971] - Analysis in control (vector only transfected HEK293T lysate) and BGN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Biglycan Antibody [NBP1-84971] - Staining of human testis shows weak to moderate cytoplasmic positivity in peritubular myoid cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Biglycan Antibody [NBP1-84971] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Biglycan Antibody [NBP1-84971] - Staining of human kidney shows moderate extracellular space positivity.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Biglycan Antibody [NBP1-84971] - Analysis in human placenta and skeletal muscle tissues. Corresponding BGN RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Biglycan Antibody [NBP1-84971] - Staining of human skeletal muscle shows no positivity in myocytes as expected.