PA5-27781

antibody from Invitrogen Antibodies

Targeting: BGN DSPG1, SLRR1A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27781

Provider

Invitrogen Antibodies

Product name

Biglycan Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T.

Concentration

0.27 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Biglycan Polyclonal Antibody (Product # PA5-27781). Sample (30 µg whole cell lysate). A: 293T. 12% SDS PAGE. Biglycan Polyclonal Antibody (Product # PA5-27781) diluted at 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot was performed using Anti-Biglycan Polyclonal Antibody (Product # PA5-27781) and a 42 kDa band corresponding to Biglycan was observed in Mouse Heart but not in Mouse Small Intestine. Tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Mouse Skeletal Muscle (Lane 2) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Biglycan Polyclonal Antibody detects BGN protein at cytosol on human Colon Epithelium by immunohistochemical analysis. Sample: Paraffin-embedded Colon Epithelium. Biglycan Polyclonal Antibody (Product # PA5-27781) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.