Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-27781 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Biglycan Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (81%), Dog (97%), Rabbit (97%), Sheep (98%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.27 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Biglycan Polyclonal Antibody (Product # PA5-27781). Sample (30 µg whole cell lysate). A: 293T. 12% SDS PAGE. Biglycan Polyclonal Antibody (Product # PA5-27781) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-Biglycan Polyclonal Antibody (Product # PA5-27781) and a 42 kDa band corresponding to Biglycan was observed in Mouse Heart but not in Mouse Small Intestine. Tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Mouse Skeletal Muscle (Lane 2) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Biglycan Polyclonal Antibody detects BGN protein at cytosol on human Colon Epithelium by immunohistochemical analysis. Sample: Paraffin-embedded Colon Epithelium. Biglycan Polyclonal Antibody (Product # PA5-27781) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.