Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-79128 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP27B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references Dietary branched-chain amino acids modulate the dynamics of calcium absorption and reabsorption in protein-restricted pigs.
Vitamin D(3) Metabolic Enzymes in the Porcine Uterus: Expression, Localization and Autoregulation by 1,25(OH)(2)D(3) In Vitro.
Habibi M, Shili CN, Sutton J, Goodarzi P, Pezeshki A
Journal of animal science and biotechnology 2022 Feb 10;13(1):15
Journal of animal science and biotechnology 2022 Feb 10;13(1):15
Vitamin D(3) Metabolic Enzymes in the Porcine Uterus: Expression, Localization and Autoregulation by 1,25(OH)(2)D(3) In Vitro.
Grzesiak M, Kaminska K, Bodzioch A, Drzewiecka EM, Franczak A, Knapczyk-Stwora K
International journal of molecular sciences 2022 Apr 2;23(7)
International journal of molecular sciences 2022 Apr 2;23(7)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CYP27B1 Polyclonal Antibody (Product # PA5-79128) and a 60kDa band corresponding to CYP27B1 was observed in all the tested cell models, and was upregulated upon LPS treatment in RAW 264.7. Whole cell lysate (30ug lysate) of HeLa (Lane 1), LNCaP (Lane 2), C2C12 (Lane 3), RAW264.7 (Lane 4), RAW264.7 treated with LPS (10µg/ml for 24hrs) (Lane 5) and THP-1 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CYP27B1 in Lane 1: rat kidney tissue lysate, Lane 2: mouse kidney tissue lysate, Lane 3: 293T whole cell lysate using 40-50 µg per well. Sample was incubated with CYP27B1 (Product # PA5-79128) at a dilution of 0.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CYP27B1 Polyclonal Antibody (Product # PA5-79128) and a 60 kDa band corresponding to CYP27B1 was observed in all the tested cell models and was upregulated upon LPS treatment in RAW 264.7. Whole cell lysate (30 µg lysate) of HeLa (Lane 1), LNCaP (Lane 2), C2C12 (Lane 3), RAW264.7 (Lane 4), RAW264.7 treated with LPS (10 µg/mL for 24 hrs) (Lane 5) and THP-1 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CYP27B1 on paraffin-embedded rat kidney tissue. Sample was incubated with CYP27B1 polyclonal antibody (Product# PA5-79128).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CYP27B1 on paraffin-embedded human kidney cancer tissue. Sample was incubated with CYP27B1 polyclonal antibody (Product# PA5-79128).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Representative micrographs of CYP27B1 immunofluorescent localization within porcine uterus on days 2-5 ( a ), 10-12 ( b ), 15-16 ( c ) and 18-20 ( d ) of the estrous cycle. Immunoreactive proteins were visualized using a Cy3 detection system (red). Nuclei were counterstained with DAPI (blue). Positive signal ( arrows ) was found in the cytoplasm of luminal (LE) and glandular (GE) epithelial cells of the endometrium, and myocytes within whole myometrial layer (only a circular myometrium (CM) is presented herein). Negative control ( c lower inset) was obtained by the replacement of primary antibody by non-immune rabbit IgG. S, stroma; asterisks (*), blood vessels. Bar = 50 um.