F54519

antibody from NSJ Bioreagents

Targeting: CYP27B1 CYP1, P450c1, PDDR, VDD1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54519

Provider

NSJ Bioreagents

Product name

CYP27B1 Antibody

Antibody type

Polyclonal

Description

This highly specific CYP27B1 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human and rat samples.

Reactivity

Human, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.05 ml, 0.2 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the CYP27B1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of rat kidney lysate with CYP27B1 antibody. Predicted molecular weight ~57 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human kidney tissue with CYP27B1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human HeLa cells with CYP27B1 antibody; Blue=isotype control, Green= CYP27B1 antibody.