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ImmunohistochemistryAntibody data
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-26065 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP27B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Hypercalcemia Owing to Overproduction of 1,25-Dihydroxyvitamin D(3) in Fetal Lung Adenocarcinoma: Case Report.
Mast cells express CYP27A1 and CYP27B1 in epithelial skin cancers and psoriasis.
Saito T, Ishida M, Kusabe M, Utsumi T, Maru N, Matsui H, Taniguchi Y, Kurata T, Kurokawa H, Imada T, Tsuta K, Tsukaguchi H, Murakawa T
JTO clinical and research reports 2021 Aug;2(8):100204
JTO clinical and research reports 2021 Aug;2(8):100204
Mast cells express CYP27A1 and CYP27B1 in epithelial skin cancers and psoriasis.
Kaukinen A, Pelkonen J, Harvima IT
European journal of dermatology : EJD 2015 Nov-Dec;25(6):548-55
European journal of dermatology : EJD 2015 Nov-Dec;25(6):548-55
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CYP27B1 in formalin fixed and paraffin embedded human kidney tissue. Samples were incubated with CYP27B1 polyclonal antibody (Product # PA5-26065) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of 293 cells using a CYP27B1 polyclonal antibody (Product # PA5-26065) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Immunohistochemical staining of CYP27B1 and schematic presentation of the pathophysiology of vitamin D 3 -mediated hypercalcemia. Immunostaining for CYP27B1 (PA5-26065, Thermo Fisher Scientific, Rockford, IL) revealed a strong positivity in alveolar macrophages (surrounded by broken line), ( A ) weak positivity in tumor cells, and ( B ) strong positivity in macrophages in granulomas within the dissected lymph nodes (below the dotted line). ( C ) Another control case with pulmonary adenocarcinoma revealed CYP27B1 negativity. ( D ) In the presented case, hypercalcemia might be induced by extrarenal CYP27B1-mediated 1,25(OH) 2 D 3 overproduction (thick gray arrows). 1,25(OH) 2 D 3 , 1,25-dihydroxyvitaminD 3 ; 25(OH)D 3 , 25-hydroxyvitamin D 3 ; Ca, calcium.
